Pulsating Electromagnetic Field Stimulates mRNA Expression of Bone Morphogenetic Protein-2 and -4
Pulsating Electromagnetic Field Stimulates mRNA Expression of Bone Morphogenetic Protein-2 and -4
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DOI：
10.1177/00220345940730100401
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发表时间：
1994-10
影响因子：
7.6
通讯作者：
Masazumi Nagai;Minoru Ota
中科院分区：
文献类型：
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作者：
Masazumi Nagai;Minoru Ota
研究了脉动电磁场对鸡胚颅盖骨骨形态发生蛋白-2和-4 mRNA表达的影响。从胚胎发生开始（第 0 天），鸡胚就在连续产生的脉动电磁场中孵化，峰值为 3.5 毫特斯拉（平均值：2 毫特斯拉），振动频率为 15 Hz。对照小鸡在正常磁场中孵育。 Northern印迹分析显示骨形态发生蛋白-2和-4的mRNA在颅骨中表达。通过狭缝印迹杂交对mRNA表达进行定量分析。磁场增强了两种 mRNA 的表达。这种增强在较年轻的鸡胚（第 15 天 > 第 17 天）中更为明显，而在 19 天大的胚胎中没有观察到显着的变化。这些结果表明磁场的骨诱导效应至少部分是由骨形态发生蛋白-2和-4介导的。
The effects of a pulsating electromagnetic field on mRNA expression of bone morphogenetic protein-2 and -4 in chick embryonic calvaria were examined. From the onset of embryogenesis (Day 0), chick embryos were incubated in a continuously generated pulsating electromagnetic field with a peak of 3.5 milli-Tesla (mean: 2 milli-Tesla) and vibration at 15 Hz. Control chicks were incubated in a normal magnetic field. Northern-blot analysis showed that the mRNAs of bone morphogenetic protein-2 and -4 were expressed in the calvaria. Quantitative analysis of the mRNA expressions was done by means of slot-blot hybridization. The magnetic field enhanced the expressions of both mRNAs. The enhancements were more pronounced in younger chick embryos (Day 15 > Day 17), and no significant change was observed in the 19-day-old embryos. These results indicate that osteo-inductive effects of the magnetic field were mediated at least in part by bone morphogenetic protein-2 and -4.