Pre-analytical and biological variability in circulating interleukin 6 in healthy subjects and patients with rheumatoid arthritis
Pre-analytical and biological variability in circulating interleukin 6 in healthy subjects and patients with rheumatoid arthritis
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DOI：
10.1080/13547500701615017
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发表时间：
2008-01-01
期刊：
影响因子：
2.6
通讯作者：
Johansen, Julia S.
中科院分区：
文献类型：
--
作者：
Knudsen, Lene S.;Christensen, I. B. J.;Johansen, Julia S.
白介素6是炎症反应中的关键分子，可能是类风湿性关节炎(RA)的有用生物标志物。目的是确定分析变异性，健康受试者的参考区间，以及健康受试者和类风湿关节炎患者血清和血浆IL-6的长期和短期变化。采用酶联免疫吸附试验检测血清和血浆IL-6水平。IL-6浓度不取决于所使用的抗凝剂的类型，也不取决于采样和处理之间的3小时延迟或反复冻融循环。318例健康人血浆和血清IL-6中位数分别为1.3pgml(-1)(0.33-26)和1.4pgml(-1)(0.25-23)。27例健康受试者血浆IL-6在1个月内、6个月和12个月后重复测定的中位变异系数分别为27%、31%和26%。在3年期间，血清IL-6没有明显的长期变化(14%，p=0.33)。运动(骑自行车)可提高健康受试者血清IL-6水平，但对RA患者无明显影响。总而言之，循环中的IL-6在样本处理方面是稳定的，并且随着时间的推移几乎没有变化。IL-6浓度的变化60%(生物变异性的2倍)可能反映疾病活动性的变化，而不仅仅是分析前或正常的生物变异性。
Interleukin (IL)-6, a key player in the inflammatory response, may be a useful biomarker in rheumatoid arthritis (RA). The aim was to determine analytical variability, a reference interval in healthy subjects, and long- and short-term variation in serum and plasma IL-6 in healthy subjects and RA patients. An enzyme-linked immunosorbent assay from RD was used for determination of serum and plasma IL-6. The IL-6 concentration did not depend on the type of anticoagulant used or the 3-h time delay between sampling and processing or repeated freeze-thaw cycles. The median plasma and serum IL-6 in 318 healthy subjects were 1.3 pg ml(-1) (range 0.33-26) and 1.4 pg ml(-1) (range 0.25-23), respectively. The median coefficient of variation in plasma IL-6 in 27 healthy subjects during 1 month, and repeated after 6 and 12 months were 27%, 31% and 26%, respectively. No significant long-term changes were observed in serum IL-6 over a 3-year period (14%, p=0.33). Exercise (cycling) increased serum IL-6 in healthy subjects but not in RA patients. In conclusion, circulating IL-6 is stable regarding sample handling and shows little variation over time. Changes in IL-6 concentrations 60% (2 times the biological variation) are likely to reflect changes in disease activity and not only pre-analytical or normal biological variability.