Effect of sodium houttuyfonate on symptom pattern of lung-Qi deficiency in rats induced by bacterialbiofilm infection.
Effect of sodium houttuyfonate on symptom pattern of lung-Qi deficiency in rats induced by bacterialbiofilm infection.
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DOI：
10.1016/s0254-6272(17)30007-9
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发表时间：
2016-12
影响因子：
2.6
通讯作者：
Wu Daqiang;Hu Weifeng;Duan Qiangjun;Cheng Huijuan
中科院分区：
文献类型：
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作者：
Wu Daqiang;Hu Weifeng;Duan Qiangjun;Cheng Huijuan
目的观察氟磺酸钠（SH）对细菌生物被膜感染所致大鼠气虚证的体内抑制作用。根据该模式，进行中医治疗。本研究所用的证型为大鼠肺气虚证，由铜绿假单胞菌（P. aeruginosa）（两株）经鼻插管滴注，在气道形成细菌生物被膜，结合寒冷和疲劳刺激诱导而成。观察各治疗组和对照组大鼠的形态、体重、脾脏和胸腺指数、血气、肺支气管组织病理学及细胞因子的变化。与模型组相比，SH治疗组小鼠的体重、脾脏指数和胸腺指数均显著升高。SH治疗组的O2分压高于模型组，CO2分压低于模型组。此外，我们还发现SH治疗组的支气管肺组织切片未显示出模型组的典型病理变化。结论在铜绿假单胞菌生物被膜感染的早期，应用SH可预防肺气虚证的发生。
OBJECTIVETo investigate thein vivoinhibitory effects of sodium houttuyfonate (SH) on symptom pattern ofQi-deficiency in rats induced by infection of bacterial biofilm on rat respiratory tract.METHODSSymptom pattern is a term used in Traditional Chinese Medicine (TCM) to define a cluster of symptoms in a medical condition. Based on the pattern, TCM therapies are administered. The symptom pattern used in this study was lung-Qideficiency pattern identified in rats, which was induced by nasal intubation drip of Pseudomonas aeruginosa (P. aeruginosa) (two strains) to form bacterial biofilm on airway combined with stimulation of cold and fatigue. We measured the variations of the symptoms of the pattern, weight, spleen and thymus index, blood gas, lung bronchial tissue pathology and cytokine of rat in different treatments and control groups.RESULTSThe rats of SH-treatment groups had not showed typical symptoms comparing with model group in the early stage of infection. The weight, spleen and thymus index of the SH-treatment groups were significantly higher comparing with untreated model group. The SH-treatment groups also showed higher O2partial pressure and lower CO2partial pressure than model group. Furthermore, we found that the bronchopulmonary section of SH-treatment groups not showed typical pathogenic variation in model group. The comparison of cytokine concentration in different groups indicated that SH could prevent the over-production of cytokine to reduce the inflammation occurrence.CONCLUSIONIn the early stage of airway infection by biofilm of P. aeruginosa, application of SH can prevent the occurrence of lung-Qideficiency pattern.