Hippocampal Reactivation Extends for Several Hours Following Novel Experience
Hippocampal Reactivation Extends for Several Hours Following Novel Experience
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DOI：
10.1523/jneurosci.1950-18.2018
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发表时间：
2019-01-30
影响因子：
5.3
通讯作者：
Diba, Kamran
中科院分区：
文献类型：
--
作者：
Giri, Bapun;Miyawaki, Hiroyuki;Diba, Kamran
新的记忆被认为是巩固了几个小时的任务后睡眠。海马细胞组装的再激活或“重放”已被提出为这一过程提供了一个关键机制。然而，先前的研究表明，这种重放仅限于任务后睡眠的前10-30分钟，这表明它在记忆巩固中的作用有限。我们进行了长时间的记录在睡眠和行为的雄性大鼠和应用的方法来评估重新激活的神经元对以及在较大的合奏，同时控制继续激活合奏已经存在于任务前的睡眠（“preplay”）。我们发现，细胞组件重新激活长达10小时，半最大时间尺度类似于6小时，在睡眠后的新的经验，即使校正的前戏。我们进一步证实了在其他使用新环境中行为的数据集中，大鼠在任务后睡眠中的类似延长的再激活。相比之下，我们在熟悉的环境中看到了有限的睡眠行为。总的来说，我们的研究结果调和重放的持续时间与归因于细胞记忆巩固的时间尺度，并为重放在记忆中的不可或缺的作用提供了强有力的支持。
New memories are believed to be consolidated over several hours of post-task sleep. The reactivation or "replay" of hippocampal cell assemblies has been proposed to provide a key mechanism for this process. However, previous studies have indicated that such replay is restricted to the first 10-30 min of post-task sleep, suggesting that it has a limited role in memory consolidation. We performed long-duration recordings in sleeping and behaving male rats and applied methods for evaluating the reactivation of neurons in pairs as well as in larger ensembles while controlling for the continued activation of ensembles already present during pre-task sleep ("preplay"). We found that cell assemblies reactivate for up to 10 h, with a half-maximum timescale of similar to 6 h, in sleep following novel experience, even when corrected for preplay. We further confirmed similarly prolonged reactivation in post-task sleep of rats in other datasets that used behavior in novel environments. In contrast, we saw limited reactivation in sleep following behavior in familiar environments. Overall, our findings reconcile the duration of replay with the timescale attributed to cellular memory consolidation and provide strong support for an integral role of replay in memory.