REI-1 Is a Guanine Nucleotide Exchange Factor Regulating RAB-11 Localization and Function in C. elegans Embryos.
REI-1 Is a Guanine Nucleotide Exchange Factor Regulating RAB-11 Localization and Function in C. elegans Embryos.
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DOI：
10.1016/j.devcel.2015.09.013
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发表时间：
2015-10
影响因子：
11.8
通讯作者：
A. Sakaguchi;Miyuki Sato;Katsuya Sato;K. Gengyo-Ando;Tomohiro Yorimitsu;J. Nakai;Taichi Hara;Ken Sato;Ken Sato
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文献类型：
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作者：
A. Sakaguchi;Miyuki Sato;Katsuya Sato;K. Gengyo-Ando;Tomohiro Yorimitsu;J. Nakai;Taichi Hara;Ken Sato;Ken Sato
小的GTPase Rab11动态地改变其位置来调节各种细胞过程，如内吞循环、分泌和胞质分裂。然而，我们对其上游监管机构的了解仍然有限。在这里，我们确定RAB-11相互作用蛋白-1(REI-1)是秀丽线虫RAB-11的一个独特的鸟嘌呤核苷酸交换因子(GEF)家族。虽然REI-1及其人类同系物SH3结合蛋白5不包含任何已知的Rab-Global结构域，但它们在体外对Rab11显示了很强的Global活性。REI-1在胚系中表达，并与RAB-11共定位于高尔基体晚期膜上。REI-1的缺失特异性地损害了RAB-11在高尔基体晚期的定位和胚胎内内体的循环，并进一步减少了RAB-11在卵裂沟中的分布，从而导致胞质分裂延迟。这些结果表明，REI-1是一种全球环境基金，专门调节RAB-11在早期胚胎中的定位和功能。
The small GTPase Rab11 dynamically changes its location to regulate various cellular processes such as endocytic recycling, secretion, and cytokinesis. However, our knowledge of its upstream regulators is still limited. Here, we identify the RAB-11-interacting protein-1 (REI-1) as a unique family of guanine nucleotide exchange factors (GEFs) for RAB-11 inCaenorhabditis elegans. Although REI-1 and its human homolog SH3-binding protein 5 do not contain any known Rab-GEF domains, they exhibited strong GEF activity toward Rab11 in vitro. InC. elegans, REI-1 is expressed in the germline and co-localizes with RAB-11 on the late-Golgi membranes. The loss of REI-1 specifically impaired the targeting of RAB-11 to the late-Golgi compartment and the recycling endosomes in embryos and further reduced the RAB-11 distribution to the cleavage furrow, which resulted in cytokinesis delay. These results suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos.