Association of intracellular and synaptic organization in cochlear inner hair cells revealed by 3D electron microscopy.
Association of intracellular and synaptic organization in cochlear inner hair cells revealed by 3D electron microscopy.
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DOI：
10.1242/jcs.170761
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发表时间：
2015-07-15
影响因子：
4
通讯作者：
Forge A
中科院分区：
文献类型：
--
作者：
Bullen A;West T;Moores C;Ashmore J;Fleck RA;MacLellan-Gibson K;Forge A
对细胞结构进行建模以满足细胞功能的方式是细胞生物学的一个知之甚少的方面。为了解决这个问题，我们研究了细胞的细胞结构与高度专业化的组织，耳蜗内毛细胞（IHC），使用多层次的三维（3D）电子显微镜分析。我们发现，IHC表面上的突触末端分布与细胞形状相关，以及包含细胞核下区域的高度组织化的膜和线粒体网络的分布。这个网络是并列的一个人口的小泡，这代表了一个潜在的新来源的神经递质囊泡补充的突触。通过高分辨率成像确定了构成该组织基础的细胞器之间的结构联系。总之，这些结果描述了一个细胞包围网络的膜和线粒体存在于IHC，支持有效的编码和传输的听觉信号。这种技术也有可能澄清其他细胞类型的功能专门的细胞结构。总结：3D电子显微镜重建了耳蜗内毛细胞核下区域的高度组织化结构，该区域支持突触功能。
The ways in which cell architecture is modelled to meet cell function is a poorly understood facet of cell biology. To address this question, we have studied the cytoarchitecture of a cell with highly specialised organisation, the cochlear inner hair cell (IHC), using multiple hierarchies of three-dimensional (3D) electron microscopy analyses. We show that synaptic terminal distribution on the IHC surface correlates with cell shape, and the distribution of a highly organised network of membranes and mitochondria encompassing the infranuclear region of the cell. This network is juxtaposed to a population of small vesicles, which represents a potential new source of neurotransmitter vesicles for replenishment of the synapses. Structural linkages between organelles that underlie this organisation were identified by high-resolution imaging. Taken together, these results describe a cell-encompassing network of membranes and mitochondria present in IHCs that support efficient coding and transmission of auditory signals. Such techniques also have the potential for clarifying functionally specialised cytoarchitecture of other cell types. Summary: 3D electron microscopy reconstructs the highly organised structure of the infranuclear region of the cochlear inner hair cell, which supports synaptic functions.