Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease
Increased interleukin 8 expression in the colon mucosa of patients with inflammatory bowel disease
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DOI：
10.1136/gut.38.2.216
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发表时间：
1996-02-01
期刊：
影响因子：
24.5
通讯作者：
Gross, V
中科院分区：
文献类型：
--
作者：
Daig, R;Andus, T;Gross, V
为探讨白细胞介素8(IL8)在克罗恩病和溃疡性结肠炎中的表达是否存在差异，并确定其合成的主要部位，本研究采用酶联免疫吸附试验(EL ISA)和原位杂交法对克罗恩病和溃疡性结肠炎患者的粘膜活检标本中的IL8进行了分析。用双抗体夹心法检测38例正常对照组、8例炎症对照组、55例克罗恩病活检标本(26例)和67例溃疡性结肠炎活检标本(35例)的IL-8水平。用特异性IL8RNA探针进行原位杂交，检测标本中IL8mRNA的表达。在肉眼可见炎症的克罗恩病标本中，IL8蛋白显著升高(中位数为118pg/标本，p
To test whether there is a difference in the expression of interleukin 8 (IL8) between Crohn's disease and ulcerative colitis and to determine the main site of its synthesis this study analysed IL8 in mucosal biopsy specimens of patients with Crohn's disease and ulcerative colitis by enzyme linked immunosorbent assay (ELISA) and by in situ hybridisation. IL8 was measured by ELISA in 38 normal control patients, eight inflammatory control patients, 55 Crohn's disease biopsy specimens (26 patients), and 67 ulcerative colitis biopsy specimens (35 patients). IL8 mRNA was determined in samples by in situ hybridisation using a specific IL8 RNA probe. IL8 protein was significantly increased in macroscopically inflamed specimens of Crohn's disease (median 118 pg/specimen, p