Ultrasensitive telomerase activity detection by telomeric elongation controlled surface enhanced Raman scattering.
Ultrasensitive telomerase activity detection by telomeric elongation controlled surface enhanced Raman scattering.
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DOI：
10.1002/smll.201301372
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发表时间：
2013-12
期刊：
影响因子：
13.3
通讯作者：
S. Zong;Zhuyuan Wang;Hui Chen;Yiping Cui
中科院分区：
文献类型：
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作者：
S. Zong;Zhuyuan Wang;Hui Chen;Yiping Cui
端粒酶被认为是癌症诊断和治疗中有价值的生物标志物和治疗靶点，这就迫切需要开发快速有效的端粒酶检测方法。提出了一种基于表面增强拉曼散射(SERS)的端粒延伸控制SERS(TEC-SERS)超灵敏端粒酶检测方法。TEC-SERS方法不仅提供了前所未有的高灵敏度，而且避免了繁琐的聚合酶链式反应程序。检出限为≈，比以前报道的方法低2-3个数量级。TEC-SERS方法灵敏度高、操作简便，可作为一种常规的端粒酶检测方法，极大地促进基于端粒酶的恶性肿瘤超早期诊断和抗癌药物的快速筛选。
Telomerase is now considered to be a valuable biomarker and therapeutic target in the diagnosis and treatment of cancerous diseases, which brings an urgent need in the development of fast and efficient telomerase detection strategies. Here, a new surface enhanced Raman scattering (SERS) based protocol using telomeric elongation controlled SERS (TEC-SERS) effect for the ultrasensitive telomerase detection is presented. The TEC-SERS protocol not only provides an unprecedented high sensitivity but also avoids laborious PCR procedures. The detection limit is ≈2-3 orders of magnitude lower than those of previously reported methods. This highly sensitive and straightforward TEC-SERS protocol can be developed as a routine telomerase detection method, which would greatly facilitate the telomerase based ultra-early diagnosis of malignant tumors and the fast screening of anti-cancer drugs.