Nociceptor-Enriched Genes Required for Normal Thermal Nociception.
Nociceptor-Enriched Genes Required for Normal Thermal Nociception.
复制标题
DOI：
10.1016/j.celrep.2016.06.003
复制
发表时间：
2016-07-12
期刊：
影响因子：
8.8
通讯作者：
Tracey WD Jr
中科院分区：
文献类型：
--
作者：
Honjo K;Mauthner SE;Wang Y;Skene JHP;Tracey WD Jr
在这里，我们描述了一个有针对性的反向遗传筛选果蝇幼虫的热伤害感受基因。使用激光捕获显微切割和伤害性和非伤害性神经元的微阵列分析，我们确定了275个伤害性感受器富集基因。然后，我们用伤害感受器特异性RNAi和热伤害感受测定来测试富集基因的功能。针对14个基因的组织特异性RNAi引起不敏感的热伤害感受，而针对22个基因的组织特异性RNAi引起超敏感的热伤害感受。以前未分类的基因被命名为耐热性（即。锅炉工、火焰舞者、烤箱手套、三脚架、thawb和掩体装备）或热敏性（点火器、黑火柴、桉树、primacord、喷气燃料、雷管、汽油、烟雾报警器和jetboil）。不敏感的伤害感受表型往往与严重减少分支的伤害感受器神经突起和超支化树突中看到的两个过敏的情况下。我们发现的许多基因在哺乳动物中是保守的。
Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (ie. boilerman, fire dancer, oven mitt, trivet, thawb and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyperbranched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals.