Upregulated INHBA expression is associated with poor survival in gastric cancer
Upregulated INHBA expression is associated with poor survival in gastric cancer
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DOI：
10.1007/s12032-010-9766-y
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发表时间：
2012-03-01
期刊：
影响因子：
3.4
通讯作者：
Peng, Zhi-Hai
中科院分区：
文献类型：
--
作者：
Wang, Quan;Wen, Yu-Gang;Peng, Zhi-Hai
表达芯片被广泛用于研究人类癌症的候选分子靶点。而基因集富集分析（GSEA）尚未在中国人胃癌（GC）中进行全基因组表达特征的筛选。为了获得GC的新分子靶标，进行GSEA分析。在本研究中，GSEA被用来挑选我们的数据库中的差异表达基因集。配对组织样本（n = 48）的总RNA和包含132个配对组织的组织微阵列用于进一步验证INHBA的表达水平及其与临床病理因素的校正。通过qPCR和免疫染色分析筛选并进一步证实胃癌中上调的INHBA表达。INHBA表达的增加与肿瘤直径和肿瘤浸润深度显著相关。INHBA表达水平较高的患者无病生存率较短。通过GSEA分析，可以有效地获得新的GC分子靶点。INHBA可能是GC的一个较差的生存指标。
Expression microarrays are widely used for investigating the candidate molecular targets in human cancer. While genome-wide expression signatures screened by gene set enrichment analysis (GSEA) were not performed in Chinese gastric cancer (GC). To gain new molecular targets for GC, GSEA analysis was performed. In the present study, GSEA were used to pick out differentially expressed gene sets of our database. Total RNA of paired tissue samples (n = 48) and a tissue microarray containing 132 paired tissues were used to further validate expression levels of INHBA and its correction with clinicopathological factors. Upregulated INHBA expression in gastric cancer was screened and further confirmed by qPCR and immunostaining analysis. Increased INHBA expression was significantly correlated with the diameter of cancer and depth of tumor invasion. Patients with higher expression levels of INHBA had a shorter disease-free survival rate. It was effective to gain new molecular targets for GC by GSEA analysis. INHBA may be a poor survival indicator of GC.