Clones of B lymphocytes in individual follicles of the bursa of Fabricius
Clones of B lymphocytes in individual follicles of the bursa of Fabricius
复制标题
法氏囊单个滤泡中 B 淋巴细胞的克隆
DOI：
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发表时间：
1985
影响因子：
5.4
通讯作者：
Anne‐Marie Rünbeek
中科院分区：
文献类型：
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作者：
J. Pink;O. Vainio;Anne‐Marie Rünbeek
为了发现单个法氏囊是否含有B淋巴细胞克隆，我们估计了两种类型的鸡嵌合体中单个毛囊中的淋巴样细胞前体细胞的数量。第一种是通过建立胚胎绒毛尿囊膜血管之间的共生连接而产生的。在这种情况下，很可能在正常发育期间，大多数卵泡由不止一个但不到十个前体细胞组成。然而，在第二种类型的嵌合体中，由正常法氏囊细胞重组的经环磷酰胺处理的小鸡，重组法氏囊中的大多数滤泡是克隆的(它们的淋巴细胞来自单一的前体细胞)。单个卵泡可以很容易地从重组鸟的法氏囊中分离出来，在B细胞发育的研究中应该是有用的。
To discover whether individual bursal follicles can contain clones of B lymphocytes, we estimated the numbers of lymphoid cell precursors populating single follicles in two types of chicken chimera. The first type was produced by establishing parabiotic connections between blood vessels of embryo chorioallantoic membranes. Under these conditions, and most likely during normal development, most follicles are populated by more than one, but less than ten, precursor cells. However, in a second type of chimera, a cyclophosphamide‐treated chick reconstituted with normal bursal cells, most follicles in the reconstituted bursa are clonal (their lymphocytes are derived from a single precursor cell). Individual follicles can readily be isolated from bursae of reconstituted birds and should be useful in studies of B cell development.