PERFORMANCE OF AN AUTOMATED-SYSTEM FOR CAPILLARY MICROINJECTION INTO LIVING CELLS
PERFORMANCE OF AN AUTOMATED-SYSTEM FOR CAPILLARY MICROINJECTION INTO LIVING CELLS
复制标题
DOI：
10.1016/0165-022x(88)90062-0
复制
发表时间：
1988-08-01
影响因子：
--
通讯作者：
PEPPERKOK, R
中科院分区：
文献类型：
--
作者：
ANSORGE, W;PEPPERKOK, R
描述了一种用于细胞核和细胞质注射的自动化毛细管显微注射系统。该系统已经过DNA、RNA和蛋白质样品的测试。毛细管的运动与精确的细胞定位和注射时间由计算机控制。这是第一个自动化显微注射系统，允许注射超过1500细胞每小时与最低限度的实际培训，注射体积更可重复性和细胞损伤较少时，与标准的手动注射技术相比。注射细胞的回收精确到1%以内，而不会在细胞支持物上产生复杂和费力的定向标记。成功注射的细胞的数量很容易以很高的精度确定，并且结果的统计评估的误差减小到最小。开发了用于提取、处理和储存注射毛细管的标准化程序。
An automated capillary microinjection system for nuclear and cytoplasmic injections into cells is described. The system has been tested with samples of DNA, RNA and proteins. Movements of the capillary with precise cell positioning and time of injections are controlled by a computer. This first automated microinjection system allows injection of more than 1500 cells per hour with a minimum of practical training, volumes injected are more reproducible and cells are less damaged when compared with the standard manual injection technique. Retrieval of the injected cells is accurate to within 1% without complicated and laborious produced orientation marks on the cell support. The number of successfully injected cells is easily determined with great accuracy and the error of the statistical evaluation of the results is reduced to a minimum. Standardized procedures for pulling, handling and storage of the injection capillaries were developed.