Hexavalent chromium reduction by Pannonibacter phragmitetus BB isolated from soil under chromium-containing slag heap
Hexavalent chromium reduction by Pannonibacter phragmitetus BB isolated from soil under chromium-containing slag heap
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DOI：
10.1080/10934520902784690
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Chen, Yue H.
中科院分区：
文献类型：
--
作者：
Chai, Li Y.;Huang, Shun H.;Chen, Yue H.
本研究的目的是寻找一个土著铬（VI）还原菌，可以有效地用于铬（VI）污染的土壤修复。结果表明，从我国某钢铁合金厂铬渣堆下土壤中分离到一株具有较强还原Cr（VI）能力的菌株。在24 h内可完全还原500 mg L-1Cr（VI）。根据16 S rRNA基因序列分析和相似性分析，将该菌株鉴定为芦苇泛酸杆菌（Pannibacter phragmitetus），并将其命名为BB菌株。扫描电子显微镜（SEM）图像显示，在500 mg L-1Cr（VI）胁迫下，芦苇BB细胞表面保持完整，细胞未破裂。透射电子显微镜（TEM）的图案表明，铬（VI）的还原产物都绑定到细胞的外表面和分散在培养基中，从而表明铬（VI）的还原发生在细胞外。通过能量色散X射线（EDX）进行的元素分析显示，Cr是包含还原产物的主要元素。此外，X射线光电子能谱（XPS）证实了Cr（VI）还原产物为Cr（III）化合物。
The objective of this study was to find an indigenous Cr(VI)-reducing bacterium that can effectively be used for Cr(VI) remediation in the contaminated soils. The results showed that one isolate from soil under a chromium-containing slag heap at a steel-alloy factory in China had a strong ability of reducing Cr(VI). It can completely reduce 500 mg L-1 Cr (VI) within 24 h. Based on 16S rRNA gene sequence and similarity analysis, this isolate was identified as Pannonibacter phragmitetus and assigned as strain BB. Images of scanning electron microscopy (SEM) indicated that the cell surface of P. phragmitetus BB remained intact without cell rupture under 500 mg L-1 Cr (VI) stress. The transmission electron microscopy (TEM) patterns showed that the Cr(VI) reduction products were both bound to the outer surface of the cells and dispersed in the culture medium, thereby suggesting that the reduction of Cr (VI) occurred extracellularly. Elemental analysis by energy dispersive X-ray (EDX) revealed that Cr was the major element comprising the reduction product. Furthermore, X-ray photoelectron spectroscope (XPS) verified that the Cr(VI) reduction product was Cr(III) compounds.