Luminal stability of insulin-like growth factors I and II in developing rat gastrointestinal tract.
Luminal stability of insulin-like growth factors I and II in developing rat gastrointestinal tract.
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DOI：
10.1097/00005176-199802000-00012
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发表时间：
1998-02
影响因子：
2.9
通讯作者：
R. Rao;A. Philipps;Catherine S. Williams;David M. McCracken;O. Koldovský
中科院分区：
文献类型：
--
作者：
R. Rao;A. Philipps;Catherine S. Williams;David M. McCracken;O. Koldovský
BACKGROUND Insulin-like growth factors (IGF)-I and -II are present in milk of a number of mammalian species. The stability of IGF-I and -II in the intestinal lumen was investigated by measuring the proteolytic degradation of 125I-labeled IGF-I and IGF-II by rat (suckling and adult) intestinal luminal flushings in vitro. METHODS Degradation of 125I-labeled IGF-I and IGF-II was assessed by measuring the generation of acid-soluble radioactivity and the reduction of the amounts of peak activity (gel filtration). Degradation was confirmed by measuring the loss of immunoreactivity and receptor activity. RESULTS Incubation of 125I-IGF-I with midjejunal luminal flushings from 12-day-old suckling rats generated acid-soluble radioactivity in a time- and dose-(flushing) dependent manner, whereas incubation of 125I-IGF-II produced only minor amounts of acid-soluble radioactivity. Degradation activity in luminal flushings from adult rat intestine was several times greater than that in luminal flushings from suckling rats. Degradation of 125I-IGF-II was several times lower than that of 125I-IGF-I in the intestinal luminal flushings from suckling and adult rats. The rate of decrease in immunoprecipitable 125I-IGF-I was considerably lower than the rate of decrease in receptor-active radioactivity. Except for immunoreactivity, analyses of acid-precipitable, peak-A, and receptor-active radioactivities demonstrate that IGF-II is relatively more stable than IGF-I in luminal flushings of suckling rat duodenum, jejunum, and midjejunum. CONCLUSIONS These results show that the stability of IGF in the gastrointestinal lumen depends on the age of the animal and the segment of the gastrointestinal tract, as well as on the peptide isoform.
