Enhanced proteoglycan deposition in the airway wall of atopic asthmatics
Enhanced proteoglycan deposition in the airway wall of atopic asthmatics
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DOI：
10.1164/ajrccm.160.2.9809040
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发表时间：
1999-08-01
影响因子：
24.7
通讯作者：
Ludwig, M
中科院分区：
文献类型：
--
作者：
Huang, J;Olivenstein, R;Ludwig, M
细胞外基质(ECM)在气道壁的沉积增加有助于观察到哮喘患者的气道壁重构。虽然胶原蛋白的改变已经被很好地描述，但对ECM的其他成分，特别是蛋白多糖(PG)的变化知之甚少。取自7名轻度特应性哮喘患者和6名正常对照组的支气管内活检。组织在OCT中封闭，并在异戊烷中冷冻。切片进行免疫组织化学染色，以检测富含亮氨酸的小颗粒、鲁米肯、大聚糖、核心蛋白和纤维调素，以及大颗粒硫酸软骨素的抗体。我们计算了上皮下层的阳性染色面积，校正了基底膜的长度。Lumcan、Biglycan和Verscan在所有组中主要分布在气道壁上皮下层。与对照组相比，哮喘组PG沉积显著增加。此外，哮喘患者PG免疫反应程度与气道反应性呈显著正相关(鲁米肯：r=-0.77，p<；0.05；Biglycan：r=-0.76，p<；0.05；versican：r=-0.74，p=0.06)。我们的结果提示PGs可能在哮喘的气道壁重构中发挥作用，从而在气道力学中发挥作用。
Increased extracellular matrix (ECM) deposition in the airway wall contributes to the airway wall remodeling observed in asthmatics. Although alterations in collagen have been well described, less is known about changes in other components of the ECM, particularly proteoglycans (PGs). Endobronchial biopsies were obtained from seven patients with mild atopic asthma and six normal control subjects. Tissues were blocked in OCT and frozen in isopentane. Sections were immunostained with antibodies for the small leucine-rich PGs, lumican, biglycan, decorin, and fibromodulin and for versican, a large chondroitin sulfate PG. We calculated the area of positive staining in the subepithelial layer, correcting for basement membrane length. Lumican, biglycan, and versican were localized predominantly in the subepithelial layer of the airway wall in all groups. PG deposition was significantly increased in asthmatics as compared with that in control subjects. Furthermore, the degree of PG immunoreactivity was significantly correlated with airway responsiveness in the asthmatics (lumican; r = -0.77, p < 0.05; biglycan: r = -0.76, p < 0.05; versican: r = -0.74, p = 0.06). Our results suggest that PGs may play a role in airway wall remodeling and thereby, airway mechanics in asthma.