Investigation of cell-surface interactions using chemical gradients formed from plasma polymers.
Investigation of cell-surface interactions using chemical gradients formed from plasma polymers.
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DOI：
10.1016/j.biomaterials.2007.09.026
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发表时间：
2008
期刊：
影响因子：
14
通讯作者：
M. Zelzer;R. Majani;J. Bradley;F. Rose;M. Davies;M. Alexander
中科院分区：
文献类型：
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作者：
M. Zelzer;R. Majani;J. Bradley;F. Rose;M. Davies;M. Alexander
本文报道了应用表面化学梯度来研究哺乳动物细胞与合成表面的相互作用，并研究了梯度某些部分的细胞反应是否与等效化学的均匀表面上的细胞反应相同。使用扩散控制等离子体聚合技术形成的梯度可以研究细胞对单个样品上多种不同化学物质的反应。在玻璃盖玻片上形成从疏水性等离子体聚合己烷 (ppHex) 到更亲水性等离子体聚合烯丙胺 (ppAAm) 的表面化学梯度，以前用于控制 3D 组织工程支架内的细胞密度。进行表面表征以确定水接触角 (WCA)、元素组成、涂层厚度和化学梯度的形貌。在陡梯度和浅梯度上培养 3T3 成纤维细胞后评估细胞反应。成纤维细胞优先在 ppAAm (WCA∼60°) 上粘附和增殖，显示出朝向疏水性 ppHex (WCA∼93°) 的细胞密度逐渐降低。在均匀 ppAAm 表面上进行的实验表明，与梯度样品相比，细胞密度存在显着差异。粘附到表面的细胞的初始数量被确认为均匀和分级 ppAAm 样品之间的差异，并且假设这种差异与不同的细胞间信号传导过程和/或这两种样品格式上周围细胞产生的更多蛋白质有关。
This paper reports on the application of surface chemical gradients to study mammalian cell interactions with synthetic surfaces and investigates if the cell response on certain parts of the gradient is the same as that on uniform surfaces of equivalent chemistry. The gradients, formed using a diffusion-controlled plasma polymerisation technique, were fabricated such that cell response to a large range of different chemistries on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised hexane (ppHex) to a more hydrophilic plasma polymerised allylamine (ppAAm), previously used to control cell density within 3D tissue-engineering scaffolds, were formed on glass coverslips. Surface characterisation was carried out to determine water contact angles (WCA), elemental composition, coating thickness and topography of the chemical gradients. Cell response was assessed following culture of 3T3 fibroblasts on both steep and shallow gradients. Fibroblasts adhered and proliferated preferentially on ppAAm (WCA∼60°) showing a gradual decreasing cell density towards the hydrophobic ppHex (WCA∼93°). Experiments on a uniform ppAAm surface revealed that there was a significant difference in cell density when compared to the gradient samples. The initial number of cells that adhered to the surface was confirmed as the difference between the uniform and graduated ppAAm samples, and it is assumed that this difference relates to different cell–cell signalling processes and/or greater protein production from surrounding cells on these two samples formats.