Nanofabrication for the analysis and manipulation of membranes.
Nanofabrication for the analysis and manipulation of membranes.
复制标题
用于膜分析和操作的纳米制造。
DOI：
10.1007/s10439-011-0479-y
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发表时间：
2012
影响因子：
3.8
通讯作者：
Craighead,HaroldG
中科院分区：
文献类型：
--
作者：
Kelly,ChristopherV;Craighead,HaroldG
纳米纤维的最新进展和应用使得能够在亚微米尺度上表征和控制生物膜。本文综述了纳米纤维在膜组分的纳米级观察、图案化、分选和浓缩中的应用。活细胞上的膜是许多基本细胞过程的必要组成部分，这些过程天然地包含膜脂质和蛋白质的纳米级重排。纳米纤维已经推进了这些理解，例如，通过在固定细胞上的扫描探针的尖端处提供30 nm分辨率的膜蛋白与金属增强的荧光。通过将荧光激发光限制在纳米级金属孔中，在60 nm分辨率下观察到活细胞上高浓度的自然扩散单分子。在膜介导的信号传导过程中质膜上的横向重组已被检查响应于信号触发分子的图案化和流动性的纳米级变化。此外，膜组件已被分离，浓缩，并通过芯片上的电泳和微流体方法提取。纳米纤维提供了许多检查和操纵膜的方法，以更好地理解膜过程以及将膜应用于其他生物物理方法。
Recent advancements and applications of nanofabrication have enabled the characterization and control of biological membranes at submicron scales. This review focuses on the application of nanofabrication towards the nanoscale observing, patterning, sorting, and concentrating membrane components. Membranes on living cells are a necessary component of many fundamental cellular processes that naturally incorporate nanoscale rearrangement of the membrane lipids and proteins. Nanofabrication has advanced these understandings, for example, by providing 30 nm resolution of membrane proteins with metal-enhanced fluorescence at the tip of a scanning probe on fixed cells. Naturally diffusing single molecules at high concentrations on live cells have been observed at 60 nm resolution by confining the fluorescence excitation light through nanoscale metallic apertures. The lateral reorganization on the plasma membrane during membrane-mediated signaling processes has been examined in response to nanoscale variations in the patterning and mobility of the signal-triggering molecules. Further, membrane components have been separated, concentrated, and extracted through on-chip electrophoretic and microfluidic methods. Nanofabrication provides numerous methods for examining and manipulating membranes for both greater understandings of membrane processes as well as for the application of membranes to other biophysical methods.