Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
复制标题
DOI：
10.1111/jre.12352
复制
发表时间：
2016-12-01
影响因子：
3.5
通讯作者：
Kawanami, M.
中科院分区：
文献类型：
--
作者：
Ogawa, K.;Miyaji, H.;Kawanami, M.
背景与目的：纳米生物陶瓷在生物医学领域的应用正在研究中。我们制造了一种再生支架，包括I型胶原蛋白和β-磷酸三钙（β-TCP）纳米颗粒。成纤维细胞生长因子-2（FGF-2）是一种生物有效的信号分子，可刺激细胞增殖和伤口愈合。本研究探讨的影响，对生物活性，纳米β-TCP/胶原支架加载FGF-2，特别是对牙周组织伤口愈合。材料和方法：β-磷酸三钙粉碎成纳米级颗粒（84 nm），然后分散。通过用纳米尺寸的β-TCP分散体涂覆胶原支架的表面来制备纳米β-TCP支架。使用扫描电子显微镜、压缩测试、细胞接种和大鼠皮下植入测试表征支架。然后将纳米β-TCP支架、载FGF-2纳米β-TCP支架和未包被胶原支架植入犬单壁骨下缺损模型中。结果：扫描电镜显示TCP纳米颗粒附着于胶原纤维上，胶原纤维的厚度明显增加。与未涂覆的胶原支架相比，纳米β-TCP支架显示出更高的抗压强度和细胞相容性。大鼠皮下植入试验表明，纳米-β-TCP支架和FGF-2负载支架中浸润细胞的DNA含量分别比胶原支架高约2.8倍和3.7倍。牙周缺损模型的组织学样本显示，植入载FGF-2的纳米-β-TCP支架后，牙周组织的修复效果是胶原支架的5倍。结论：β-TCP纳米颗粒涂层大大提高了胶原支架的生物活性。含有FGF-2的纳米-β-TCP支架有望用于牙周组织工程。
Background and Objective: Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (beta-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects, on bioactivity, of a nano-beta-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing.Material and Methods: Beta-tricalcium phosphate was pulverized into nanosize particles (84 nm) and was then dispersed. A nano-beta-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nanosize beta-TCP dispersion. Scaffolds were characterized using scanning electron microscopy, compressive testing, cell seeding and rat subcutaneous implant testing. Then, nano-beta-TCP scaffold, nano-beta-TCP scaffold loaded with FGF-2 and noncoated collagen scaffold were implanted into a dog one-wall infrabony defect model. Histological observations were made at 10 d and 4 wk post-surgery.Results: Scanning electron microscopy images show that TCP nanoparticles were attached to collagen fibers. The nano-beta-TCP scaffold showed higher compressive strength and cytocompatibility compared with the noncoated collagen scaffold. Rat subcutaneous implant tests showed that the DNA contents of infiltrating cells in the nano-beta-TCP scaffold and the FGF-2-loaded scaffold were approximately 2.8-fold and 3.7-fold greater, respectively, than in the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-beta-TCP scaffold loaded with FGF-2 compared with the collagen scaffold.Conclusion: The beta-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-beta-TCP scaffolds containing FGF-2 are anticipated for use in periodontal tissue engineering.