The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis
The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis
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长非编码 RNA T5120 调节拟南芥中的硝酸盐反应和同化
DOI：
10.1111/nph.16038
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发表时间：
2019-07-29
期刊：
影响因子：
9.4
通讯作者：
Wang, Yong
中科院分区：
文献类型：
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作者：
Liu, Fei;Xu, Yiran;Wang, Yong
长链非编码RNA（longnoncodingRNA，lncRNA）在植物的许多生理过程中起着重要的调节作用。然而，它仍然是未知的lncRNA是否可以响应硝酸盐或硝酸盐调节功能。我们检测到695 lncRNA，480已知和215新的，在拟南芥幼苗根，6个显示改变表达响应硝酸盐处理，其中T5120显示最高的诱导。在拟南芥中过量表达T5120促进了对硝酸盐的响应，增强了硝酸盐的同化作用，提高了生物量和根系发育。生化和分子分析表明，NLP 7，一个主硝酸盐调节转录因子，直接绑定到T5120启动子的硝酸盐响应顺式元件（NRE）样基序和激活T5120转录。此外，T5120部分恢复了NLP 7突变体的硝酸盐信号传导和同化表型，表明T5120参与了NLP 7介导的硝酸盐调节。有趣的是，T5120的表达受硝酸盐传感器NRT1.1的调节。因此，T5120受到NLP 7和NRT1.1的调节，以调节硝酸盐信号传导。我们的工作揭示了一种新的调节机制，其中lncRNA T5120在硝酸盐调节中发挥作用，为硝酸盐信号网络提供了新的见解。重要的是，lncRNA T5120可以促进硝酸盐同化和植物生长，从而提高氮素利用效率。
Long noncoding RNAs (lncRNAs) are crucial regulators in many plant biological processes. However, it remains unknown whether lncRNAs can respond to nitrate or function in nitrate regulation. We detected 695 lncRNAs, 480 known and 215 novel, in Arabidopsis seedling roots; six showed altered expression in response to nitrate treatment, among which T5120 showed the highest induction. Overexpression of T5120 in Arabidopsis promoted the response to nitrate, enhanced nitrate assimilation and improved biomass and root development. Biochemical and molecular analyses revealed that NLP7, a master nitrate regulatory transcription factor, directly bound to the nitrate-responsive cis-element (NRE)-like motif of the T5120 promoter and activated T5120 transcription. In addition, T5120 partially restored the nitrate signalling and assimilation phenotypes of nlp7 mutant, suggesting that T5120 is involved in NLP7-mediated nitrate regulation. Interestingly, the expression of T5120 was regulated by the nitrate sensor NRT1.1. Therefore, T5120 is modulated by NLP7 and NRT1.1 to regulate nitrate signalling. Our work reveals a new regulatory mechanism in which lncRNA T5120 functions in nitrate regulation, providing new insights into the nitrate signalling network. Importantly, lncRNA T5120 can promote nitrate assimilation and plant growth to improve nitrogen use efficiency.