Direct screening of clinical specimens for multiple respiratory pathogens using the Genaco Respiratory Panels 1 and 2
Direct screening of clinical specimens for multiple respiratory pathogens using the Genaco Respiratory Panels 1 and 2
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DOI：
10.1097/01.pdm.0000210430.35340.53
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发表时间：
2006-09-01
影响因子：
--
通讯作者：
Thomas, Eva
中科院分区：
文献类型：
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作者：
Brunstein, John;Thomas, Eva
我们在此报告了一项试点研究的结果，该研究将我们临床诊断病毒学实验室目前的呼吸道病原体检测方法与Genaco呼吸道感染小组1和2进行了比较。这些检测采用了xMAP(Luminex)液体微珠共轭阵列技术，以促进对PCR和RT-PCR产物的自动检测，这为检测水平高于目前实用的传统凝胶分解或实时方法提供了可能性。在这里介绍的研究中，我们使用Genaco专家小组同时筛查了先前分析过的临床标本(鼻咽冲洗液)中的21种重要病原体。我们的结果表明，Genaco板达到或超过了我们当前方法的灵敏度和特异度，尽管允许检测比当前诊断实验室实践实际更广泛的感染性病原体。此外，Genaco小组提供了关于单一样本中存在多种呼吸道病原体的数据，否则在大多数情况下会遗漏这些数据。据我们所知，这项研究代表了在常规诊断环境中对标准临床标本进行的这些小组的首次试验。
We report here on the results of a pilot study comparing our clinical diagnostic virology laboratory's current methods of respiratory pathogen detection against the Genaco Respiratory Infections Panels 1 and 2. These assays employ xMap (Luminex) liquid phase bead conjugated array technology to facilitate automated detection of PCR and RT-PCR products, which provides potential for levels of assay multiplexing above those currently practical with either conventional gel-resolved or real-time methods. In the study presented here we used the Genaco panels to simultaneously screen previously analyzed clinical specimens (nasopharyngeal washings) for twenty-one important pathogens. Our results indicate the Genaco panels met or exceeded our current methods' sensitivity and specificity although allowing for detection of a wider range of infectious agents than practical by current diagnostic laboratory practices. In addition, the Genaco panels provided data on the presence of multiple respiratory pathogens in single specimens, which would otherwise be missed in most instances. To our knowledge, this study represents the first trial of these panels on standard clinical specimens in a routine diagnostic setting.