Validation of a Vertical Progression Porcine Burn Model
Validation of a Vertical Progression Porcine Burn Model
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DOI：
10.1097/bcr.0b013e31822dc439
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发表时间：
2011-11-01
影响因子：
1.4
通讯作者：
Clark, Richard A. F.
中科院分区：
文献类型：
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作者：
Singer, Adam J.;Hirth, Douglas;Clark, Richard A. F.
烧伤治疗的一个主要潜在目标是限制部分至全层烧伤的进展。为了更好地测试治疗方法，作者开发并验证了一种垂直进展猪烧伤模型，在该模型中，用闭塞敷料治疗的部分厚度烧伤转化为全层烧伤，并以瘢痕和伤口收缩愈合。用150克铝条预热到70摄氏度、80摄氏度或90摄氏度，持续20或30秒，在两头幼猪的背部和侧翼造成40处接触烧伤。坏死表皮被去除，烧伤部位用聚氨脂闭塞敷料覆盖。烧伤分别于伤后1、24、48小时以及7、14、21、28天拍摄。分别在第1、4、24、48小时以及第7、28天进行全层活检。主要结果是在受伤后28天存在深度收缩的疤痕和伤口面积。次要结局是损伤深度、表皮再生和疤痕深度。采用方差分析和chi(2)检验比较不同烧伤情况下的数据。使用以下温度/接触时间组合，用铝棒创建了八个重复烧伤：70/20、70/30、80/20、80/30和90/20。烧伤愈合伴瘢痕收缩的比例为70/ 20.0%；70/30, 25%;80/20, 50%;80/30, 75%;90/20为100% （P = 0.05）。28 d时伤口面积70/ 20,8.1 cm(2)；70/30, 7.8 cm(2)；80/20, 6.6 cm(2)；80/30, 4.9 cm(2)；90/20, 4.8 cm(2) （P = .007）。80/20和80/30烧伤1小时内皮损伤深度判断损伤深度分别为皮肤厚度的36%和60%。损伤后1小时内皮细胞损伤深度与瘢痕面积呈负相关（Pearson相关r = - 0.71, P < 0.001）。将猪皮肤暴露在预热到80摄氏度的铝棒上20或30秒，最初会导致部分厚度的烧伤，当用闭塞敷料治疗时，会发展为全层损伤，并愈合时留下明显的疤痕和伤口挛缩。（中华烧伤杂志2011；32:638-646）
A major potential goal of burn therapy is to limit progression of partial-to full-thickness burns. To better test therapies, the authors developed and validated a vertical progression porcine burn model in which partial-thickness burns treated with an occlusive dressing convert to full-thickness burns that heal with scarring and wound contraction. Forty contact burns were created on the backs and flanks of two young swine using a 150 g aluminum bar preheated to 70 degrees C, 80 degrees C, or 90 degrees C for 20 or 30 seconds. The necrotic epidermis was removed and the burns were covered with a polyurethane occlusive dressing. Burns were photographed at 1, 24, and 48 hours as well as at 7, 14, 21, and 28 days postinjury. Full-thickness biopsies were obtained at 1, 4, 24, and 48 hours as well as at 7 and 28 days. The primary outcomes were presence of deep contracted scars and wound area 28 days after injury. Secondary outcomes were depth of injury, reepithelialization, and depth of scars. Data were compared across burn conditions using analysis of variance and chi(2) tests. Eight replicate burns were created with the aluminum bar using the following temperature/contact-time combinations: 70/20, 70/30, 80/20, 80/30, and 90/20. The percentage of burns healing with contracted scars were 70/20, 0%; 70/30, 25%; 80/20, 50%; 80/30, 75%; and 90/20, 100% (P = .05). Wound areas at 28 days by injury conditions were 70/20, 8.1 cm(2); 70/30, 7.8 cm(2); 80/20, 6.6 cm(2); 80/30, 4.9 cm(2); and 90/20, 4.8 cm(2) (P = .007). Depth of injury judged by depth of endothelial damage for the 80/20 and 80/30 burns at 1 hour was 36% and 60% of the dermal thickness, respectively. The depth of injury to the endothelial cells 1 hour after injury was inversely correlated with the degree of scar area (Pearson's correlation r = -.71, P < .001). Exposure of porcine skin to an aluminum bar preheated to 80 C for 20 or 30 seconds results initially in a partial-thickness burn that when treated with an occlusive dressing progresses to a full-thickness injury and heals with significant scarring and wound contracture. (J Burn Care Res 2011;32:638-646)