The Measurement of Intraocular Biomarkers in Various Stages of Proliferative Diabetic Retinopathy Using Multiplex xMAP Technology.
The Measurement of Intraocular Biomarkers in Various Stages of Proliferative Diabetic Retinopathy Using Multiplex xMAP Technology.
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DOI：
10.1155/2015/424783
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发表时间：
2015
影响因子：
1.9
通讯作者：
Topolcan O
中科院分区：
文献类型：
--
作者：
Rusnak S;Vrzalova J;Sobotova M;Hecova L;Ricarova R;Topolcan O
目的。使用多重 xMAP 技术测定不同严重程度的增殖性糖尿病视网膜病变 (PDR) 患者的眼内生长因子和细胞因子水平。方法。一项前瞻性队列研究，纳入了 56 名患者的 61 只眼睛，根据 PDR 的严重程度分为 3 组。第 1 组患者是那些出现 PDR 且无需重复手术干预的患者；第2组患者出现反复玻璃体出血；第3组的患者患有难治性新生血管性青光眼。测量眼内液中促血管生成因子、抗血管生成因子、炎症因子和神经营养因子的浓度。结果还与老年性白内障手术前 50 名患者的 50 只眼睛（对照组）测量的因子水平进行了比较。结果。难治性新生血管性青光眼患者（临床严重程度最高的组）的白介素 6 (IL-6)（中位数 1 37.19；中位数 3 384.74；P = .00096）、转化生长因子 β 1 (TGFβ-1)（中位数 1 49.00；中位数 3 414.40；P = .0017）和血管内皮生长水平较高与其他 PDR 患者相比，因子 (VEGF)（中位数 1 211.62；中位数 3 352.82；P = .0454）。结论。我们的研究结果表明 IL-6、TGFβ-1 和 VEGF 的水平与 PDR 的严重程度相关。
Purpose. To determine the intraocular levels of growth factors and cytokines in patients with various degrees of severity of proliferative diabetic retinopathy (PDR) using multiplex xMAP technology. Methods. A prospective cohort study of 61 eyes from 56 patients who were divided into 3 groups based on the severity of PDR. Patients in group number 1 are those who presented PDR with no need of repeated surgical intervention; patients in group number 2 had repeated vitreous bleeding; and patients in group number 3 had refractory neovascular glaucoma. The concentrations of proangiogenic, antiangiogenic, inflammatory, and neurotrophic factors were measured in intraocular fluid. The results were also compared with levels of factors measured in 50 eyes from 50 patients prior to senile cataract surgery (control group). Results. Patients with refractory neovascular glaucoma (the highest clinical severity group) had higher levels of interleukin 6 (IL-6) (median1 37.19; median3 384.74; P = .00096), transforming growth factor beta 1 (TGFβ-1) (median1 49.00; median3 414.40; P = .0017), and vascular endothelial growth factor (VEGF) (median1 211.62; median3 352.82; P = .0454) compared with other PDR patients. Conclusions. Results of our study imply that levels of IL-6, TGFβ-1, and VEGF correlate with the severity of PDR.