Functional annotation of circRNAs of tea leaves during the infection of the pathogen of tea leaf spot, Lasiodiplodia theobromae
Functional annotation of circRNAs of tea leaves during the infection of the pathogen of tea leaf spot, Lasiodiplodia theobromae
复制标题
茶树叶斑病病原菌可可二孢菌感染过程中茶叶circRNA的功能注释
DOI：
10.1094/phyto-05-21-0184-a
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Chen Z.
中科院分区：
文献类型：
--
作者：
Yang Y. Y.;Jiang X. Y.;Shi J. Y.;Wang Y.;Huang H. L.;Yang Y. Q.;Li D. X.;Jiang S. L.;Jiang S. L.;Wang D. L.;Chen Z.
茶树叶斑病是一种严重影响茶叶产量和品质的重要病害，由茶树毛二孢（Lasiodiplodia theobromae）引起。O. Kuntze）离开。通过对病原菌侵染后茶叶中环状RNA（circRNA）的分析，可以加深对寄主-病原菌互作机制的认识。在这项研究中，高性能测序的circRNA从C。sinensis福鼎大白茶叶片感染L.使用Illumina HiSeq 4000平台进行可可碱分析。总共有192个和153个来自茶叶的差异表达的circRNA在感染L.可可碱。基因本体分析表明，差异表达的circRNA宿主基因的DNA结合显着富集。在特定数据库（Sgenes）中注释的具有显著差异表达的基因是σ因子E亚型1、三酰甘油脂肪酶SDP 1、DNA指导的RNA聚合酶III亚基2、WRKY转录因子WRKY 24和无义转录物调节因子1同源物。京都基因和基因组百科全书分析表明，参与植物-病原体相互作用途径的显著富集的circRNA宿主基因是钙调蛋白结构域蛋白激酶5同种型1、可能的WRKY转录因子33、含U盒结构域的蛋白35、可能的失活受体样蛋白激酶At 3g 56050、WRKY转录因子WRKY 24、促分裂原活化蛋白激酶YODA、SGT 1、蛋白质DGS 1侵染茶树叶片中circRNA的功能注释可可碱将为宿主-病原体相互作用的未来研究提供有价值的资源。
Tea leaf spot, caused byLasiodiplodia theobromae, is an important disease that can seriously decrease the production and quality of tea (Camellia sinensis(L.) O. Kuntze) leaves. The analysis of circular RNA (circRNA) in tea leaves after infection by the pathogen could improve understanding about the mechanism of host–pathogen interactions. In this study, high-performance sequencing of circRNA fromC. sinensisFuding-dabaicha leaves that had been infected withL. theobromaewas conducted using the Illumina HiSeq 4000 platform. In total, 192 and 153 differentially expressed circRNAs from tea leaves were significantly up- and downregulated, respectively, after infection withL. theobromae. A gene ontology analysis indicated that the differentially expressed circRNA-hosting genes for DNA binding were significantly enriched. The genes with significantly differential expressions that were annotated in the specified database (Sgenes) were σfactor E isoform 1,triacylglycerol lipase SDP1,DNA-directed RNA polymerase III subunit 2,WRKY transcription factor WRKY24, andregulator of nonsense transcripts 1 homolog. A Kyoto Encyclopedia of Genes and Genomes analysis indicated that the significantly enriched circRNA-hosting genes involved in the plant–pathogen interaction pathway wereCalmodulin-domain protein kinase 5 isoform 1,probable WRKY transcription factor 33,U-box domain-containing protein 35,probable inactive receptor-like protein kinase At3g56050,WRKY transcription factor WRKY24,mitogen-activated protein kinase kinase kinase YODA,SGT1, and proteinDGS1. Functional annotation of circRNAs in tea leaves infected byL. theobromaewill provide a valuable resource for future research on host–pathogen interactions.