Organization of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca)
Organization of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca)
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DOI：
10.1007/s10126-003-0016-6
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发表时间：
2004-05-01
影响因子：
3
通讯作者：
Nishida, S
中科院分区：
文献类型：
--
作者：
Machida, RJ;Miya, MU;Nishida, S
南极磷虾是南极沃茨最重要的生态和商业动物之一，我们测定了南极磷虾（Euphausia superba，甲壳纲：软甲纲）线粒体基因组的近全DNA序列。所有的基因组序列通过使用长聚合酶链反应（PCR）的基因扩增进行纯化，并且随后将产物用作模板，用于使用引物步移策略的直接测序或使用甲壳类通用引物的巢式PCR。虽然由于技术困难，我们无法确定基因组的一部分，但测序的14，606 bp长的位置包含所有13个蛋白质编码基因，22个转运RNA基因中的19个，大亚基以及一部分小亚基核糖体RNA基因。观察到3个转运RNA基因（tRNA（、）（Cys）、tRNA（Tyr）和tRNA（Trp））和2个亮氨酸tRNA基因的基因重排。
We determined the nearly complete DNA sequence of the mitochondrial genome of Antarctic krill Euphausia superba (Crustacea: Malacostraca), one of the most ecologically and commercially important zoo-plankters in Antarctic waters. All of the genome sequences were purified by gene amplification using long polymerase chain reaction (PCR), and the products were subsequently used as templates for either direct sequencing using a primer-walking strategy or nested PCR with crustacea-versatile primers. Although we were unable to determine a portion of the genome owing to technical difficulties, the sequenced position, 14,606 bp long, contained all of the 13 protein-coding genes, 19 of the 22 transfer RNA genes, and the large subunit as well as a portion of the small subunit ribosomal RNA genes. Gene rearrangement was observed for 3 transfer RNA genes (tRNA(,)(Cys) tRNA(Tyr), and tRNA(Trp)) and the 2 leucine tRNA genes.