Effects of silver and gold nanoparticles of different sizes in human pulmonary fibroblasts
Effects of silver and gold nanoparticles of different sizes in human pulmonary fibroblasts
复制标题
DOI：
10.3109/15376516.2015.1025347
复制
发表时间：
2015-01-01
影响因子：
3.2
通讯作者：
Morales, Paloma
中科院分区：
文献类型：
--
作者：
Avalos, Alicia;Isabel Haza, Ana;Morales, Paloma
银和金纳米颗粒（Ag-AuNP）是目前生产最多的纳米材料之一。因此，应调查与人类接触 Ag-AuNPs 相关的危害，以促进风险评估过程。特别是，由于在处理这些纳米粒子的过程中发生了Ag-AuNPs的肺部暴露，因此有必要评估肺细胞的毒性反应。本研究的目的是评估不同尺寸的银（4.7 和 42 nm）和金纳米颗粒（30、50 和 90 nm）对人肺成纤维细胞（HPF）的体外毒性机制。通过观察细胞活力和氧化应激参数来评估毒性。数据显示，AgNPs 诱导的细胞毒性具有尺寸依赖性，而三种尺寸的 AuNPs 显示出相似的细胞毒性。 4.7nm 的银纳米颗粒比大的银纳米颗粒和 AuNP 的毒性要大得多。然而，用抗氧化剂 N-乙酰基-L-半胱氨酸进行预处理可以保护 HPF 细胞免受 Ag-AuNP 的处理。氧化应激参数显示活性氧水平显着增加、谷胱甘肽水平下降以及超氧化物歧化酶轻微但不具有统计学意义的失活，表明氧化应激的产生。因此，在加工和配制 Ag-AuNP 直至最终成品时必须小心谨慎。
Silver and gold nanoparticles (Ag-AuNPs) are currently some of the most manufactured nanomaterials. Accordingly, the hazards associated with human exposure to Ag-AuNPs should be investigated to facilitate the risk assessment process. In particular, because pulmonary exposure to Ag-AuNPs occurs during handling of these nanoparticles, it is necessary to evaluate the toxic response in pulmonary cells. The aim of this study was to evaluate the in vitro mechanisms of toxicity of different sizes of silver (4.7 and 42 nm) and gold nanoparticles (30, 50 and 90 nm) in human pulmonary fibroblasts (HPF). The toxicity was evaluated by observing cell viability and oxidative stress parameters. Data showed that AgNPs-induced cytotoxicity was size-dependent, whereas the AuNPs of the three sizes showed similar cytotoxicity. Silver nanoparticles of 4.7nm were much more toxic than the large silver nanoparticles and the AuNPs. However, the pre-treatment with the antioxidant, N-acetyl-L-cysteine, protected HPF cells against treatment with Ag-AuNPs. The oxidative stress parameters revealed significant increase in reactive oxygen species levels, depletion of glutathione level and slight, but not statistically significant inactivation of superoxide dismutase, suggesting generation of oxidative stress. Hence, care has to be taken while processing and formulating the Ag-AuNPs till their final finished product.