Thermo-Plasmonic Trapping of Living Cyanobacteria on a Gold Nanopyramidal Dimer Array: Implications for Plasmonic Biochips
Thermo-Plasmonic Trapping of Living Cyanobacteria on a Gold Nanopyramidal Dimer Array: Implications for Plasmonic Biochips
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DOI：
10.1021/acsanm.0c02071
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发表时间：
2020-10-23
影响因子：
5.9
通讯作者：
Tsuboi, Yasuyuki
中科院分区：
文献类型：
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作者：
Naka, Shota;Shoji, Tatsuya;Tsuboi, Yasuyuki
随着等离子体生物芯片的发展，我们研究了蓝藻(CB)在金纳米结构上的激光捕获，在金纳米结构中集成了纳米二聚体阵列。在近红外(808 Nm)激光照射样品溶液后，CB细胞立即被收集、捕获并永久固定在纳米结构的照射区域。将大约15个CB细胞固定成环状。由于气泡的产生始终是CB固定所必需的，固定不是由于等离子体增强的光学力，而是由于流体动力压力调制和气泡周围的Marangoni对流。气泡是由开水的光热效应产生的。其固定机制类似于气泡纳米光刻。我们用荧光显微光谱学检测了CB细胞的生物活性和活性。我们的方法将适用于开发基于活细胞的生物设备。
With implication of developments of plasmonic biochips, we investigated laser trapping of cyanobacteria (CB) on a gold nanostructure, where a nanopyramidal dimer array was integrated. Upon near-infrared (808 nm) laser irradiation of the sample solution, CB cells were immediately collected, trapped, and permanently fixed onto the irradiated area of the nanostructure. About 15 CB cells were fixed to form a ring pattern. Because bubble generation was always necessary for CB fixation, the fixation was not because of a plasmon-enhanced optical force but because of hydrodynamic pressure modulation and Marangoni convection around the bubble. The bubble was generated by a photothermal effect to boil water. The fixation mechanism would be analogous to that of bubble nanolithography. We examined bioactivity and viability of CB cells using fluorescence microspectroscopy. Our method would be applicable to develop living-cell-based biodevices.