Characterization of Vaginal Microbial Community Dynamics in the Pathogenesis of Incident Bacterial Vaginosis, a Pilot Study
Characterization of Vaginal Microbial Community Dynamics in the Pathogenesis of Incident Bacterial Vaginosis, a Pilot Study
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DOI：
10.1097/olq.0000000000001821
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发表时间：
2023-08-01
影响因子：
3.1
通讯作者：
Muzny, Christina A.
中科院分区：
文献类型：
--
作者：
Elnaggar, Jacob H.;Lammons, John W.;Muzny, Christina A.
背景
尽管已有60多年的研究，但细菌性阴道炎（BV）的病因仍存在争议。在这项初步研究中，我们采用鸟枪法宏基因组测序来描述初发性BV（iBV）发生前阴道微生物群落的变化。
方法
对一群基线时阴道微生物组健康（无Amsel标准，Nugent评分为0 - 3且无阴道加德纳菌形态类型）的非裔美国女性进行为期90天的随访，她们每天自行采集阴道标本以检测iBV（连续至少2天Nugent评分为7 - 10）。对4名女性的部分阴道标本进行鸟枪法宏基因组测序，在iBV诊断前12天每隔一天进行一次。通过Kraken2和bioBakery 3工作流程对测序数据进行分析，并将标本分类为不同的群落状态类型。进行定量聚合酶链反应，以比较读数计数与细菌丰度的相关性。
结果
在iBV发生前，参与者体内越来越多地检测到常见的与BV相关的细菌，如阴道加德纳菌、二路普雷沃菌和阴道芬海斯菌。线性模型表明，在iBV发生前，阴道加德纳菌和阴道芬海斯菌的相对丰度显著增加，而乳酸杆菌属的相对丰度随时间下降。乳酸杆菌属的减少与乳酸杆菌噬菌体的存在相关。我们观察到在iBV发生前几天细菌黏附因子基因富集。细菌读数计数与通过定量聚合酶链反应测量的丰度之间也存在显著相关性。
结论
这项初步研究描述了iBV发生前阴道群落的动态变化，并确定了可能参与iBV发病机制的关键细菌类群和机制。
BackgroundDespite more than 60 years of research, the etiology of bacterial vaginosis (BV) remains controversial. In this pilot study, we used shotgun metagenomic sequencing to characterize vaginal microbial community changes before the development of incident BV (iBV).MethodsA cohort of African American women with a baseline healthy vaginal microbiome (no Amsel criteria, Nugent score 0-3 with no Gardnerella vaginalis morphotypes) were followed for 90 days with daily self-collected vaginal specimens for iBV (& GE;2 consecutive days of a Nugent score of 7-10). Shotgun metagenomic sequencing was performed on select vaginal specimens from 4 women, every other day for 12 days before iBV diagnosis. Sequencing data were analyzed through Kraken2 and bioBakery 3 workflows, and specimens were classified into community state types. Quantitative polymerase chain reaction was performed to compare the correlation of read counts with bacterial abundance.ResultsCommon BV-associated bacteria such as G. vaginalis, Prevotella bivia, and Fannyhessea vaginae were increasingly identified in the participants before iBV. Linear modeling indicated significant increases in G. vaginalis and F. vaginae relative abundance before iBV, whereas the relative abundance of Lactobacillus species declined over time. The Lactobacillus species decline correlated with the presence of Lactobacillus phages. We observed enrichment in bacterial adhesion factor genes on days before iBV. There were also significant correlations between bacterial read counts and abundances measured by quantitative polymerase chain reaction.ConclusionsThis pilot study characterizes vaginal community dynamics before iBV and identifies key bacterial taxa and mechanisms potentially involved in the pathogenesis of iBV.