Identification of the aromatic L-amino acid decarboxylase gene expression in various mice tissues and its modulation by immobilization stress in stellate ganglia
Identification of the aromatic L-amino acid decarboxylase gene expression in various mice tissues and its modulation by immobilization stress in stellate ganglia
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DOI：
10.1016/j.neuroscience.2004.04.005
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Kvetnansky, R
中科院分区：
文献类型：
--
作者：
Kubovcakova, L;Krizanova, O;Kvetnansky, R
尽管各种应激刺激对儿茶酚胺生物合成酶基因表达、活性和免疫反应蛋白的影响已被广泛研究，但对l -二羟基苯丙氨酸脱羧生成多巴胺的芳香族l -氨基酸脱羧酶（AADC）知之甚少。我们专注于在各种小鼠组织中鉴定AADC mRNA和免疫蛋白，并在选定的小鼠神经元组织（肾上腺髓质、交感星状神经和颈神经节）和非神经元组织（肝、脾、肾和心脏的所有四个部分）中检测AADC mRNA和免疫蛋白。令人惊讶的是，尽管我们未能在小鼠胸腺、肺和腹部脂肪中检测到AADC mRNA，但我们发现肺和腹部脂肪中都存在AADC免疫蛋白。我们还验证了假设，单次或多次固定应激是否会影响小鼠星状神经节中的AADC mRNA或免疫蛋白水平。我们发现，单次固定应激暴露不影响AADC mRNA或免疫蛋白水平，而多次固定应激使星状神经节中AADC mRNA和免疫蛋白水平显著升高。芳香l -氨基酸脱羧酶通常不被认为是限制调节儿茶酚胺的生物合成。然而，我们的数据表明这种酶可能参与了反复应激小鼠星状神经节中儿茶酚胺生物合成的调节。(c) 2004 ibro。Elsevier Ltd.出版。版权所有。
Despite of the fact that the impact of various stressful stimuli on catecholamine biosynthetic enzyme gene expression, activity and immunoreactive protein has been intensively studied, less is known about the aromatic L-amino acid decarboxylase (AADC), the enzyme, which catalyzes decarboxylation Of L-dihydroxyphenylalanine to dopamine.We focused on the identification of AADC mRNA and immunoprotein in various mice tissues and detected both in selected mice neuronal tissues (adrenal medulla, sympathetic stellate and cervical ganglia) and also in non-neuronal tissues (liver, spleen, kidney and all four parts of the heart). Surprisingly, although we failed to detect AADC mRNA in mice thymus, lungs and abdominal fat, we found presence of the AADC immunoprotein in lungs as well as in the abdominal fat.We also tested the hypothesis, whether single or repeated immobilization stress can affect the AADC mRNA or immunoprotein levels in mice stellate ganglia. We revealed that single immobilization stress exposure did not affect the AADC mRNA or immunoprotein levels, while repeated immobilization stress produced significant elevation of both, AADC mRNA and immunoprotein levels in stellate ganglia.The aromatic L-amino acid decarboxylase is generally not considered to be limiting in regulation of the catecholamine biosynthesis. However, our data suggest a possible participation of this enzyme in the regulation of catecholamine biosynthesis in stellate ganglia of repeatedly stressed mice. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.