Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China
Antibiotics, Antibiotic Resistance Genes, and Bacterial Community Composition in Fresh Water Aquaculture Environment in China
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DOI：
10.1007/s00248-015-0583-x
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
Zeng, Zhenling
中科院分区：
文献类型：
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作者：
Xiong, Wenguang;Sun, Yongxue;Zeng, Zhenling
环境抗生素耐药性对人类健康的威胁日益引起人们的关注。本研究调查了广东省水产养殖环境中抗生素（四环素类、磺胺类和氟喹诺酮类）的浓度和抗生素耐药基因（包括四环素耐药基因、磺胺类耐药基因和质粒介导的喹诺酮类耐药基因）的丰度，并分析了细菌群落组成。磺胺甲氧嘧啶，磺胺二甲嘧啶，磺胺甲恶唑，土霉素，金霉素，多西环素，环丙沙星，诺氟沙星，恩诺沙星的浓度高达446 μ g kg（-1）和98.6 ng L-1的沉积物和水样中，分别。ARG（sul 1、sul 2、sul 3、tetM、tetO、tetW、tetS、tetQ、tetX、tetB/P、qepA、oqxA、oqxB、aac（6 '）-Ib和qnrS）的相对丰度（ARG拷贝/16 S核糖体RNA（rRNA）基因拷贝）高达2.8 x 10（-2）。沉积物中的优势菌门为变形菌门、拟杆菌门和厚壁菌门，水样中的优势菌门为变形菌门、放线菌门和拟杆菌门。还观察到与病原体相关的属，如不动杆菌属、弓形杆菌属和梭菌属。本研究对我国水产养殖环境中的抗生素、ARGs和细菌群落组成进行了全面调查。结果表明，鱼塘是ARG的水库，潜在的抗性和病原相关的分类群体在鱼塘中的存在可能意味着对人类健康的潜在风险。
Environmental antibiotic resistance has drawn increasing attention due to its great threat to human health. In this study, we investigated concentrations of antibiotics (tetracyclines, sulfonamides and (fluoro)quinolones) and abundances of antibiotic resistance genes (ARGs), including tetracycline resistance genes, sulfonamide resistance genes, and plasmid-mediated quinolone resistance genes, and analyzed bacterial community composition in aquaculture environment in Guangdong, China. The concentrations of sulfametoxydiazine, sulfamethazine, sulfamethoxazole, oxytetracycline, chlorotetracycline, doxycycline, ciprofloxacin, norfloxacin, and enrofloxacin were as high as 446 mu g kg(-1) and 98.6 ng L-1 in sediment and water samples, respectively. The relative abundances (ARG copies/16S ribosomal RNA (rRNA) gene copies) of ARGs (sul1, sul2, sul3, tetM, tetO, tetW, tetS, tetQ, tetX, tetB/P, qepA, oqxA, oqxB, aac(6')-Ib, and qnrS) were as high as 2.8 x 10(-2). The dominant phyla were Proteobacteria, Bacteroidetes, and Firmicutes in sediment samples and Proteobacteria, Actinobacteria and Bacteroidetes in water samples. The genera associated with pathogens were also observed, such as Acinetobacter, Arcobacter, and Clostridium. This study comprehensively investigated antibiotics, ARGs, and bacterial community composition in aquaculture environment in China. The results indicated that fish ponds are reservoirs of ARGs and the presence of potential resistant and pathogen-associated taxonomic groups in fish ponds might imply the potential risk to human health.