ROLE OF LYMPHOCYTES IN SENSITIZATION OF RATS TO RENAL HOMOGRAFTS
ROLE OF LYMPHOCYTES IN SENSITIZATION OF RATS TO RENAL HOMOGRAFTS
复制标题
DOI：
10.1084/jem.122.2.347
复制
发表时间：
1965-01-01
影响因子：
15.3
通讯作者：
GOWANS, JL
中科院分区：
文献类型：
--
作者：
STROBER, S;GOWANS, JL
为研究小淋巴细胞在大鼠同种肾移植致敏中的作用，采用两种离体大鼠肾脏灌流方法：将胸导管细胞悬于人工培养液或血液中，体外灌流，然后将灌流液注射到与淋巴细胞供体同系的大鼠体内，再将其注射到离体大鼠肾脏。用从活体大鼠的股动脉流出并返回到股静脉的血液对肾脏进行体内灌注。通过应用来自肾供体的皮肤同种移植物并在6天时对上皮坏死进行评分来评估灌注液赋予受体的致敏程度。体外实验表明，亲本品系胸导管细胞，其中已通过F1杂交肾可以赋予亲本大鼠对F1皮肤移植物的敏感性。用放射性标记的淋巴细胞进行的几次灌注表明，注射的细胞迁移到受体的淋巴结和脾脏。标记的大嗜派洛宁细胞偶尔在受体的脾脏和淋巴结中观察到，这表明这些细胞是由注射的细胞产生的。虽然体外血液灌流表明同种肾移植物在1小时内就可致敏宿主，但体内灌流表明约需5 - 12小时。体外致敏更快可能是由于体外条件提供了淋巴细胞与肾血管内皮细胞更频繁接触的机会。
In order to study the role of blood-borne small lymphocytes in the sensitization of rats to renal homografts two techniques for the perfusion of isolated rat kidneys were employed: the in vitro perfusion of kidneys with thoracic duct cells suspended in either an artificial medium or in blood; the perfusates were then injected into rats syngeneic with the lymphocyte donors; the in vivo perfusion of kidneys with blood issuing from the femoral artery and returning to the femoral vein of living rats. The degree of sensitization conferred on the recipients by the perfusates was assessed by applying a skin homograft from the kidney donor and scoring the epithelial necrosis at 6 days. The in vitro experiments indicated that parental strain thoracic duct cells, which had passed through an F1 hybrid kidney could confer upon a parental rat sensitivity to an F1 skin graft. Several perfusions with radioactively labelled lymphocytes showed that the injected cells migrated to the lymph nodes and spleen of the recipients. Labelled large pyroninophilic cells were occasionally seen in the spleen and lymph nodes of recipients, and it was suggested that these had arisen from the injected cells. Although the in vitro perfusions with blood indicated that renal homo-grafts might sensitize their hosts within 1 hour, the in vivo perfusions suggested that about 5 to 12 hours were required. The more rapid sensitization in vitro was possibly due to the more frequent opportunity for contact between lymphocytes and kidney vascular endothelium which was afforded by the conditions in vitro.