Methylation changes in faecal DNA:: a marker for colorectal cancer screening?
Methylation changes in faecal DNA:: a marker for colorectal cancer screening?
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DOI：
10.1016/s0140-6736(04)16002-9
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发表时间：
2004-04-17
期刊：
影响因子：
168.9
通讯作者：
Widschwendter, M
中科院分区：
文献类型：
--
作者：
Müller, HM;Oberwalder, M;Widschwendter, M
DNA甲基化是结直肠癌细胞中常见的分子改变。我们报告了对结直肠癌患者和对照组粪便DNA的评估，以确定这种方法的可行性、灵敏度和特异性。通过使用MethyLight分析来自三组独立患者的粪便DNA，我们确定SFRP 2甲基化是粪便样本中鉴定结直肠癌的敏感的单一DNA标记物（训练集[n=23]的敏感性90% [CI 56-100]和特异性77% [46-95];在独立测试集[n=26]中，灵敏度77%[46 -95]和特异性77% [46-95]）。遗传和表观遗传标记的组合是否能在早期阶段识别结直肠癌仍有待证实。
DNA methylation is a common molecular alteration in colorectal cancer cells. We report an assessment of faecal DNA from patients with colorectal cancer and controls to determine the feasibility, sensitivity, and specificity of this approach. By use of MethyLight analysis of faecal DNA from three independent sets of patients, we identified SFRP2 methylation as a sensitive single DNA-based marker for identification of colorectal cancer in stool samples (sensitivity 90% [CI 56-100] and specificity 77% [46-95] in the training set [n=23]; sensitivity 77% [46-95] and specificity 77% [46-95] in an independent test set [n=26]). Whether a combination of genetic and epigenetic markers will identify colorectal cancer at an early stage remains to be shown.