Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA
Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNA
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DOI：
10.1016/j.bios.2007.06.018
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发表时间：
2007-12-15
影响因子：
12.6
通讯作者：
Mandrand, Bernard
中科院分区：
文献类型：
--
作者：
Bouchet, Aurelie;Chaix, Carole;Mandrand, Bernard
利用生长在微型化石墨电极上的圆柱形导电聚吡咯的电化学性质，研制了一种新型的多检测生物传感器。我们的目标是构思一种用于生物医学诊断的灵敏、无标记和实时的DNA传感器。在第一步中，将同时带有二茂铁氧化还原标记和寡核苷酸探针的共聚物选择性地在微芯片电极上进行电寻址。然后，对它们在添加DNA靶标时的伏安响应的研究表明，通过改变二茂铁的氧化强度，杂交得到了有效的转换。利用这一技术，在人类免疫缺陷病毒和乙肝病毒靶标之间获得了良好的选择性。事实上，直接跟随杂交是可能的。互补DNA检测下限达到100 pm(30亩L 3fmol)，这代表了这样一种实用、无标记、实时的传感器的良好性能。(C)2007 Elsevier B.V.保留所有权利。
A new multidetection biosensor has been developed using the electrochemical properties of cylinder-shaped conducting polypyrrole grown on miniaturized graphite electrodes. Our objective was to conceive a sensitive, labelless and real-time DNA sensor for biomedical diagnosis. In a first step, copolymers bearing both ferrocene redox markers and oligonucleotide probes were selectively electro-addressed on microchip electrodes. Then, the study of their voltammetric response upon the addition of DNA targets revealed that the hybridization was efficiently transduced through the variation of ferrocene oxidation intensity. Using this technique, a good selectivity between Human Immunodeficiency Virus and Hepatitis B Virus targets was obtained. It was indeed possible to directly follow the hybridization. Complementary DNA detection limit reached 100 pM (3 fmol in 30 mu L), which represents a good performance for such a practical, labelless and real-time sensor. (c) 2007 Elsevier B.V. All rights reserved.