Age-dependent effects of blue light exposure on lifespan, neurodegeneration, and mitochondria physiology in Drosophila melanogaster.
Age-dependent effects of blue light exposure on lifespan, neurodegeneration, and mitochondria physiology in Drosophila melanogaster.
复制标题
DOI：
10.1038/s41514-022-00092-z
复制
发表时间：
2022-07-27
期刊：
影响因子：
--
通讯作者：
Giebultowicz, Jadwiga M
中科院分区：
文献类型：
--
作者：
Song, Yujuan;Yang, Jun;Law, Alexander D;Hendrix, David A;Kretzschmar, Doris;Robinson, Matthew;Giebultowicz, Jadwiga M
蓝光是发光器件（LED）的主要组成部分，其越来越多地存在于我们的环境中。已经有越来越多的证据表明，蓝光暴露会对体外和体内的视网膜细胞造成损伤;然而，关于蓝光对非视网膜细胞的潜在影响知之甚少。蓝光可能在生物体水平上是有害的，与视网膜效应无关，最近的研究结果表明，它会减少蠕虫和视网膜基因消融的苍蝇的寿命。在这里，我们研究了蓝光暴露对果蝇寿命的影响，发现对蓝光应激的敏感性强烈依赖于年龄。相同强度和持续时间的蓝光降低了老年果蝇的存活率，增加了神经退行性变，比年轻果蝇更明显。这些差异似乎至少部分是由线粒体呼吸功能受损引起的。我们报告，蓝光显着降低电子传递系统中的复合物II的活性，并降低年轻和老年果蝇琥珀酸脱氢酶的生化活性。此外，复合物I和复合物IV的活性随着年龄的增长而降低，ATP水平也是如此。因此，我们提出，老年果蝇对蓝光更敏感，因为光诱导的线粒体损伤增强了即使在黑暗中也会发生的与年龄相关的能量代谢障碍。综上所述，我们的研究结果表明，蓝光在生物体水平上的破坏作用具有强烈的年龄依赖性，并且与线粒体中能量产生途径的特定组分的活性降低有关。
Blue light is a predominant component of light emitting devices (LEDs), which are increasingly present in our environment. There is already accumulating evidence that blue light exposure causes damage to retinal cells in vitro and in vivo; however, much less is known about potential effects of blue light on non-retinal cells. That blue light may be detrimental at the organismal level independent from retinal effect was recently shown by findings that it reduces lifespan in worms and also in flies with genetically ablated retinas. Here, we investigated the effects of blue light exposure across the fly lifespan and found that susceptibility to blue light stress is strongly age-dependent. The blue light of the same intensity and duration reduced survival and increased neurodegeneration more significantly in old flies than in young flies. These differences appear to be caused, at least in part, by impairments of mitochondrial respiratory function. We report that blue light significantly reduces the activity of Complex II in the electron transport system and decrease the biochemical activity of succinate dehydrogenase in both young and old flies. In addition, complex I and complex IV activities are reduced by age, as are ATP levels. We therefore propose that older flies are more sensitive to blue light because the light-induced mitochondrial damage potentiates the age-related impairments in energy metabolism that occurs even in darkness. Taken together, our results show that damaging effects of blue light at the organismal level are strongly age dependent and are associated with reduced activity of specific components of energy producing pathways in mitochondria.