The distribution of Cdh20 mRNA demarcates somatotopic subregions and subpopulations of spiny projection neurons in the rat dorsolateral striatum
The distribution of Cdh20 mRNA demarcates somatotopic subregions and subpopulations of spiny projection neurons in the rat dorsolateral striatum
复制标题
Cdh20 mRNA 的分布划分了大鼠背外侧纹状体中多刺投射神经元的体位亚区和亚群
DOI：
10.1002/cne.25215
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发表时间：
2021
影响因子：
2.5
通讯作者：
Kawakami Kiyoshi
中科院分区：
文献类型：
--
作者：
Takahashi Masanori;Fukabori Ryoji;Kawasaki Hiroshi;Kobayashi Kazuto;Kawakami Kiyoshi
啮齿类动物的背外侧纹状体（DLS）在功能上被细分为身体定位亚区，这些亚区代表身体沿着背腹和前后（A-P）轴的每个部位，并通过皮质纹状体通路在感觉运动功能中起关键作用。然而，很少有人知道的空间基因表达模式和异质性的多刺投射神经元（SPNs）内的somatotopic亚区。在此，我们发现，细胞粘附分子基因Cdh 20（编码II型钙粘蛋白）在大鼠DLS腹侧区（v-DLS）覆盖内口面区和部分前肢区的离散亚区中表达。Cdh 20表达细胞定位于纹状体沿着A-P轴的中间水平的v-DLS中，可分为直接途径SPN或间接途径SPN。出乎意料的是，综合分析显示Cdh 20在大鼠DLS的SPN中表达，而在小鼠DLS或雪貂壳核（Pu）中不表达。我们的观察结果表明Cdh 20表达在大鼠DLS中特异性地划分了SPN的体细胞定位亚区和亚群，并表明在哺乳动物中v-DLS和Pu中差异表达的基因的不同调节。
The dorsolateral striatum (DLS) of rodents is functionally subdivided into somatotopic subregions that represent each body part along both the dorsoventral and anteroposterior (A‐P) axes and play crucial roles in sensorimotor functions via corticostriatal pathways. However, little is known about the spatial gene expression patterns and heterogeneity of spiny projection neurons (SPNs) within somatotopic subregions. Here, we show that the cell adhesion molecule geneCdh20, which encodes a Type II cadherin, is expressed in discrete subregions covering the inner orofacial area and part of the forelimb area in the ventral domain of the DLS (v‐DLS) in rats.Cdh20‐expressing cells were localized in the v‐DLS at the intermediate level of the striatum along the A‐P axis and could be classified as direct‐pathway SPNs or indirect‐pathway SPNs. Unexpectedly, comprehensive analysis revealed thatCdh20is expressed in SPNs in the rat DLS but not in the mouse DLS or the ferret putamen (Pu). Our observations reveal thatCdh20expression demarcates somatotopic subregions and subpopulations of SPNs specifically in the rat DLS and suggest divergent regulation of genes differentially expressed in the v‐DLS and Pu among mammals.