Dielectrophoretic sorting of particles and cells in a microsystem
Dielectrophoretic sorting of particles and cells in a microsystem
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DOI：
10.1021/ac971063b
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发表时间：
1998-05-01
影响因子：
7.4
通讯作者：
Fuhr, G
中科院分区：
文献类型：
--
作者：
Fiedler, S;Shirley, SG;Fuhr, G
存在用于在非常小的体积中探测细胞和分子事件的高度灵敏的分析技术。开发用于在这样的体积中进行有效样品处理和分离的微型工具仍然是一个挑战。迄今为止开发的大多数设备使用电泳和色谱分离方法。我们表明，也可以使用负介电泳（DEP）条件下的交流场产生的力。微型化的电极阵列被安置在一个微通道中，并由高频交流电驱动。层流携带颗粒通过电极。交流驱动器的修改改变了粒子的轨迹。我们已经在一个装置中处理了微米大小的乳胶颗粒和活的哺乳动物细胞，该装置由以下四个元件组成：一个平面漏斗，它将颗粒从1 mm宽的流集中到约50 μ m宽的射束，一个对准器，它使射束进一步变窄并起破碎颗粒聚集体的作用，一个保持的笼，它可用于捕获颗粒，以及一个可以将粒子引导到两个输出通道之一中的开关。电极由玻璃基板上的铂/钛和氧化铟锡（ITO）制成。颗粒浓度和开关可以实现线性流动速度高达约10毫米秒（-1）。这种新方法与高性能光学检测的结合为小型化流式细胞术提供了前景。
There are highly sensitive analytical techniques for probing cellular and molecular events in very small volumes. The development of microtools for effective sample handling and separation in such volumes remains a challenge. Most devices developed so far use electrophoretic and chromatographic separation methods. We show that forces generated by ac fields under conditions of negative dielectrophoresis (DEP) can also be used. Miniaturized electrode arrays are housed in a microchannel and driven with high-frequency ac. A laminar liquid flow carries particles past the electrodes. Modification of the ac drive changes the particle trajectories. We have handled latex particles of micrometer size and living mammalian cells in a device which consists of the following four elements: a planar funnel which concentrates particles from a 1-mm-wide stream to a beam of about 50-mu m width, an aligner which narrows the beam further and acts to break up particle aggregates, a held cage which can be used to trap particles, and a switch which can direct particles into one of two output channels. The electrodes are made from platinum/titanium and indium tin oxide (ITO) on glass substrates. Particle concentration and switching could be achieved for linear now velocities up to about 10 mm s(-1). The combination of this new method with highperformance optical detection offers prospects for miniaturized flow cytometry.