Intracellular delivery and ultrasonic activation of folate receptor-targeted phase-change contrast agents in breast cancer cells in vitro.
Intracellular delivery and ultrasonic activation of folate receptor-targeted phase-change contrast agents in breast cancer cells in vitro.
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DOI：
10.1016/j.jconrel.2016.09.010
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发表时间：
2016-12-10
期刊：
影响因子：
--
通讯作者：
Matsunaga TO
中科院分区：
文献类型：
--
作者：
Marshalek JP;Sheeran PS;Ingram P;Dayton PA;Witte RS;Matsunaga TO
乳腺癌是一种多样而复杂的疾病，仍然是妇女死亡的主要原因之一。需要新颖、创新的成像和治疗方法来补充现有的技术。在这项研究中，我们在体外MDA-MB-231和MCF-7乳腺癌细胞中提供了叶酸受体（FR）靶向相变造影剂（PCCAs）的细胞内递送和超声刺激激活的证据。pcca是一种脂质包被、全氟化碳填充的颗粒，其配方为纳米级液滴，能够蒸发成气体微泡，用于成像或治疗。细胞与1:1的十氟丁烷(DFB) /八氟丙烷（OFP） pcca孵育1小时，通过共聚焦显微镜成像，暴露于超声（9 MHz, MI = 1.0或1.5），超声后再次成像。两种细胞系均在细胞内观察到fr靶向的pcca，但MDA-MB-231细胞的摄取率（MI = 1.0时为93.0%,MI = 1.5时为79.5%）明显高于MCF-7细胞（MI = 1.0时为42.4%,MI = 1.5时为35.7%）。与非靶向PCCA相比，叶酸掺入使MDA-MB-231细胞的细胞内PCCA检测频率增加了45倍，MCF-7细胞的细胞内PCCA检测频率增加了7倍。胞内活化的PCCAs范围为500 nm ~ 6微米（IQR = 800 nm ~ 1.5微米），平均直径为1.15±0.59 （SD）微米。本文提出的工作证明了使用乳腺癌细胞进行PCCA细胞内传递和激活的可行性，为细胞内成像或超声治疗传递提供了新的平台。
Breast cancer is a diverse and complex disease that remains one of the leading causes of death among women. Novel, outside-of-the-box imaging and treatment methods are needed to supplement currently available technologies. In this study, we present evidence for the intracellular delivery and ultrasound-stimulated activation of folate receptor (FR)-targeted phase-change contrast agents (PCCAs) in MDA-MB-231 and MCF-7 breast cancer cells in vitro. PCCAs are lipid-coated, perfluorocarbon-filled particles formulated as nanoscale liquid droplets capable of vaporization into gaseous microbubbles for imaging or therapy. Cells were incubated with 1:1 decafluorobutane (DFB) / octafluoropropane (OFP) PCCAs for 1 hour, imaged via confocal microscopy, exposed to ultrasound (9 MHz, MI = 1.0 or 1.5), and imaged again after insonation. FR-targeted PCCAs were observed intracellularly in both cell lines, but uptake was significantly greater (p < 0.001) in MDA-MB-231 cells (93.0% internalization at MI = 1.0, 79.5% at MI = 1.5) than MCF-7 cells (42.4% internalization at MI = 1.0, 35.7% at MI = 1.5). Folate incorporation increased the frequency of intracellular PCCA detection 45-fold for MDA-MB-231 cells and 7-fold for MCF-7 cells, relative to untargeted PCCAs. Intracellularly activated PCCAs ranged from 500 nm to 6 microns (IQR = 800 nm – 1.5 microns) with a mean diameter of 1.15 ± 0.59 (SD) microns. The work presented herein demonstrates the feasibility of PCCA intracellular delivery and activation using breast cancer cells, illuminating a new platform toward intracellular imaging or therapeutic delivery with ultrasound.
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DOI：
10.1016/j.jconrel.2013.09.013
发表时间：
2013-12-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Ernsting MJ;Murakami M;Roy A;Li SD
通讯作者：
Li SD
增强载有硫酸长春新碱的叶酸缀合 PLGA-PEG 纳米颗粒在人乳腺癌中的细胞摄取
影响因子：
3.4
作者：
Chen, Jianian;Li, Shaoshun;Zhang, Yi
通讯作者：
Zhang, Yi
DOI：
10.1016/j.jphotobiol.2015.05.008
发表时间：
2015-08-01
影响因子：
5.4
作者：
Banu, Hussaina;Sethi, Dipinder Kaur;Vasanthakumar, Geetha
通讯作者：
Vasanthakumar, Geetha
DOI：
10.1186/bcr3493
发表时间：
2013-10-01
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Eccles SA;Aboagye EO;Ali S;Anderson AS;Armes J;Berditchevski F;Blaydes JP;Brennan K;Brown NJ;Bryant HE;Bundred NJ;Burchell JM;Campbell AM;Carroll JS;Clarke RB;Coles CE;Cook GJ;Cox A;Curtin NJ;Dekker LV;Silva Idos S;Duffy SW;Easton DF;Eccles DM;Edwards DR;Edwards J;Evans D;Fenlon DF;Flanagan JM;Foster C;Gallagher WM;Garcia-Closas M;Gee JM;Gescher AJ;Goh V;Groves AM;Harvey AJ;Harvie M;Hennessy BT;Hiscox S;Holen I;Howell SJ;Howell A;Hubbard G;Hulbert-Williams N;Hunter MS;Jasani B;Jones LJ;Key TJ;Kirwan CC;Kong A;Kunkler IH;Langdon SP;Leach MO;Mann DJ;Marshall JF;Martin L;Martin SG;Macdougall JE;Miles DW;Miller WR;Morris JR;Moss SM;Mullan P;Natrajan R;O'Connor JP;O'Connor R;Palmieri C;Pharoah PD;Rakha EA;Reed E;Robinson SP;Sahai E;Saxton JM;Schmid P;Smalley MJ;Speirs V;Stein R;Stingl J;Streuli CH;Tutt AN;Velikova G;Walker RA;Watson CJ;Williams KJ;Young LS;Thompson AM
通讯作者：
Thompson AM
影响因子：
12.4
作者：
Matsunaga TO;Sheeran PS;Luois S;Streeter JE;Mullin LB;Banerjee B;Dayton PA
通讯作者：
Dayton PA