Impact of the synbiotic combination of Lactobacillus casei shirota and oligofructose-enriched inulin on the fecal volatile metabolite profile in healthy subjects
Impact of the synbiotic combination of Lactobacillus casei shirota and oligofructose-enriched inulin on the fecal volatile metabolite profile in healthy subjects
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DOI：
10.1002/mnfr.201000442
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发表时间：
2011-05-01
影响因子：
5.2
通讯作者：
Verbeke, Kristin
中科院分区：
文献类型：
--
作者：
De Preter, Vicky;Ghebretinsae, Aklilu Habteab;Verbeke, Kristin
范围：假说驱动的方法主要集中在将单链脂肪酸作为合生元有益效应的介体进行量化。然而，代谢物分析策略的出现允许从自上而下的方法评估结肠代谢。在本研究中，我们评估了合生素组合对粪便代谢产物谱的影响。方法和结果：在9名健康志愿者中评价了合生素组合(干酪乳杆菌Shirota细胞+富含低聚果糖的菊糖)。在开始治疗前、治疗期间和治疗4周后，采集粪便样本。采用GC-MS技术对挥发性代谢物进行分析。应用III型测试显示，三种条件下的代谢物分布有显著不同。我们确定了三种挥发性有机化合物，乙酸酯、二甲基三硫化物和乙苯，它们受到了显著的影响。在干预过程中和干预后，醋酸盐水平上升，而二甲基三硫化物水平下降。对于乙苯，只有在共生干预期间才能观察到影响。结论：我们详细分析了干酪乳杆菌与富含低聚果糖的菊粉联合使用对发酵代谢产物的影响。我们的结果表明，糖解发酵的刺激，更重要的是，潜在有毒蛋白发酵代谢产物二甲基三硫醚和乙苯的减少也参与了这些作用。
Scope: Hypothesis-driven approaches have mainly focused on the quantification of SCFAs as mediators of beneficial effects of synbiotics. However, the emergence of metabolite profiling strategies allows to evaluate the colonic metabolism from a top-down approach. In the present study, we evaluated the impact of a synbiotic combination on fecal metabolite profiles.Methods and results: A synbiotic combination ( Lactobacillus casei Shirota cells+oligofructose-enriched inulin) was evaluated in nine healthy volunteers. Before the start, during and after 4-wk treatment, fecal samples were obtained. GC-MS technology was applied to analyze the volatile metabolites. Application of a Type III test revealed that the metabolite profiles from the three conditions were significantly different. We identified three volatile organic compounds, acetate, dimethyl trisulfide and ethyl benzene, which were significantly affected. The acetate levels increased, whereas the dimethyl trisulfide levels decreased during and after the intervention. For ethyl benzene only an effect during the synbiotic intervention period was observed.Conclusion: We report a detailed analysis of the influence of L. casei Shirota combined with oligofructose-enriched inulin on fermentation metabolites. Our results indicated a stimulation of saccharolytic fermentation and, importantly, a reduction of potentially toxic protein fermentation metabolites dimethyl trisulfide and ethyl benzene attended these effects.