Modulation of types I and III procollagen synthesis at various stages of arterial smooth muscle cell growth in vitro.
Modulation of types I and III procollagen synthesis at various stages of arterial smooth muscle cell growth in vitro.
复制标题
在体外动脉平滑肌细胞生长的各个阶段调节 I 型和 III 型前胶原合成。
DOI：
10.1016/0014-4827(84)90443-9
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发表时间：
1984
影响因子：
3.7
通讯作者：
Ehrhart,LA
中科院分区：
文献类型：
--
作者：
Holderbaum,D;Ehrhart,LA
在兔动脉平滑肌细胞（SMC）培养物中监测胶原合成。在接种平滑肌细胞后1至14天的特定时间间隔测量每个细胞的胶原合成速率和胶原合成占总蛋白质合成的百分比。还检查了条件培养培养基和细胞层中存在的 I 型和 III 型胶原蛋白的比例。接种后，细胞表现出SMC典型的群体扩张，其中生长减慢，但在细胞达到汇合后并未停止。胶原蛋白合成率（以每个细胞的[14C]羟脯氨酸表示）在汇合前细胞中高出八倍。在这些培养物中，胶原蛋白占新合成的 14 C 标记蛋白的 20% 以上，在 24 小时培养基中以三氯乙酸 (TCA) 不溶性物质的形式存在。在汇合后培养物中，这一百分比减少至合成总蛋白的 7% 左右。胶原蛋白和非胶原蛋白的合成率随着接种后时间的增加而降低。然而，胶原蛋白合成的下降速度是非胶原蛋白合成下降速度的三倍。早期培养物合成的 I 型原胶原多于 III 型原胶原。 I 型与 III 型的比例在第 3 天最高，此后到第 14 天下降。虽然两种类型的合成随着年龄的增长而减少，但 I 型下降的速度更快，导致较老的培养物分泌 III 型前胶原占主导地位。我们得出的结论是，蛋白质合成，特别是胶原蛋白合成，在数量和质量上取决于 SMC 的体外生长阶段。
Collagen synthesis was monitored in cultures of rabbit arterial smooth muscle cells (SMC). Both the rate of collagen synthesis per cell and collagen synthesis as a percent of total protein synthesis were measured at specific intervals from 1 to 14 days after inoculation of smooth muscle cells. The proportions of types I and III collagen present in the conditioned incubation medium and in the cell layer were also examined. After inoculation the cells displayed population expansion typical of SMC in which growth slowed but did not cease after the cells attained confluence. Collagen synthesis rates, expressed as [14C]hydroxyproline per cell, were eight-fold higher in preconfluent cells. In these cultures collagen accounted for more than 20% of the newly synthesized,14C-labeled protein present as trichloroacetic acid (TCA)-insoluble material in 24 h culture media. In postconfluent cultures, this percentage was reduced to about 7% of the total protein synthesized. Synthesis rates of both collagen and non-collagen protein decreased with increasing time after inoculation. However, the rate of decline of collagen synthesis was three times greater than that seen for non-collagen protein. Early cultures synthesized relatively more type I than type III procollagen. The type I to type III ratio was highest at day 3 and declined after that time to day 14. While the synthesis of both types decreased with increasing age, type I declined at a greater rate resulting in a predominance of type III procollagen secretion by older cultures. We conclude that protein synthesis in general and collagen synthesis in particular are quantitatively and qualitatively dependent upon the growth stage of SMC in vitro.