Red blood cells serve as a vehicle for PEDV transmission
Red blood cells serve as a vehicle for PEDV transmission
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红细胞是 PEDV 传播的载体。
DOI：
10.1016/j.vetmic.2021.109081
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发表时间：
2021-04-23
影响因子：
3.3
通讯作者：
Yang, Qian
中科院分区：
文献类型：
--
作者：
Li, Jianda;Yuan, Chen;Yang, Qian
作为血液中最丰富的细胞类型，红细胞 (RBC) 负责输送氧气。然而，红细胞结合病毒的机制仍然很大程度上未知。在这里，我们证明了猪流行性腹泻病毒（PEDV）是一种冠状病毒，可以劫持红细胞并引起新生仔猪的典型腹泻。在PEDV流行病学调查中，多个猪场腹泻猪的红细胞样本均呈PEDV阳性。 PEDV可通过CD71和网格蛋白介导的内吞作用与新生儿红细胞结合，其活力可维持12小时。负载PEDV的红细胞可以通过接合将病毒转移至CD3+T细胞并到达肠粘膜，在那里引起感染。最后，进一步的动物挑战表明，输注携带 PEDV 的红细胞可能会导致肠上皮细胞 (IEC) 感染和典型的腹泻症状。因此，我们的研究阐明了PEDV通过劫持红细胞引起肠道感染的机制，进一步为红细胞作为冠状病毒发病机制中病毒传播的潜在细胞的作用提供了新的见解。
As the most abundant cell type in the blood, red blood cells (RBCs) are serving for transporting oxygen. However, the mechanism by which RBCs binding virus remains largely unknown. Here, we demonstrated that porcine epidemic diarrhea virus (PEDV), a kind of coronavirus, could hijack RBCs and cause typical diarrhea in neonatal piglets. In an epidemiology investigation of PEDV, the RBCs samples from diarrheic pigs in several pig farms were found to be PEDV-positive. PEDV could bind to neonatal RBCs through CD71 and clathrin-mediated endocytosis, and its viability was maintained for 12 h. PEDV-loaded RBCs could transfer the virus to CD3+ T cells by conjugation and reach the intestine mucosa, where it caused infection. Finally, a further animal challenge revealed that transfusing with PEDV-loaded RBCs could cause intestinal epithelial cells (IECs) infection and typical diarrhea symptom. Therefore, our studies illustrated the mechanism by which PEDV could cause intestinal infection through hijacking RBCs, further providing a novel insight into the role of RBCs as potential cells for viral transmission in coronavirus pathogenesis.