Induction and Measurement of Cytotoxic T Lymphocyte Activity.
Induction and Measurement of Cytotoxic T Lymphocyte Activity.
复制标题
DOI：
10.1002/cpim.38
复制
发表时间：
2018-02-21
影响因子：
--
通讯作者：
Presby, Matthew M
中科院分区：
文献类型：
--
作者：
Wonderlich, John;Shearer, Gene;Presby, Matthew M
细胞毒性T细胞（ctl）是适应性免疫应答中重要的免疫效应细胞。有充分的证据表明，ctl在宿主对病毒和细菌细胞内病原体、肿瘤和移植组织的免疫反应中很重要。ctl的特性已经在小鼠模型中得到了广泛的研究，它们在人类环境中的作用也得到了验证。通常，这些细胞的存在与保护性免疫密切相关，因此容易测量这些细胞活性的能力是一项重要的免疫学测量。在本单元中，介绍了几种通常用于诱导CTL和测量体外和体内CTL活性的测定方法。这些测定法适用于许多实验和/或疾病模型，在体外测定法的情况下，可用于测量人类样品中的CTL活性。©2018 by John Wiley & Sons, Inc。
Cytotoxic T cells (CTLs) are important immune effector cells in the adaptive immune response. It has been well documented that CTLs are important in host immune responses to viral and bacterial intracellular pathogens, tumors, and transplanted tissues. The properties of CTLs have been studied extensively in murine models, and their roles validated in the human setting. Frequently, the presence of these cells correlates well with protective immunity, so the ability to readily measure the activity of these cells is an important immunological measurement. In this unit, several assays are described that are commonly utilized to induce CTLs and to measure CTL activity both in vitro and in vivo. These assays are adaptable to many experimental and/or disease models, and in the case of the in vitro assays can be applied to measure CTL activity in human samples. © 2018 by John Wiley & Sons, Inc.