Identification of a two-component VirR/VirS regulon in Clostridium perfringens
Identification of a two-component VirR/VirS regulon in Clostridium perfringens
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DOI：
10.1016/j.anaerobe.2009.10.003
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发表时间：
2010-06-01
期刊：
影响因子：
2.3
通讯作者：
Shimizu, Tohru
中科院分区：
文献类型：
--
作者：
Ohtani, Kaori;Hirakawa, Hideki;Shimizu, Tohru
产气荚膜梭菌是一种革兰氏阳性厌氧病原体，是人类气性坏疽的病原体，导致严重的快速组织破坏，除非立即治疗，否则可在数小时内导致死亡。已知几种毒素的产生是由涉及C中的调节RNA（VR-RNA）的双组分VirR/VirS系统控制的。产气荚膜杆菌为了阐明由VirR/VirS和VR-RNA控制的精确调控网络，进行了一系列使用VirR/VirS和VR-RNA缺陷突变体的微阵列筛选。最后，通过qRT-PCR分析，147个基因（30个单基因和21个推定的操纵子）被证实是在VirR/VirS-VR-RNA调控级联反应的控制下。发现了α-毒素、κ-毒素、透明质酸酶、唾液酸酶、荚膜多糖合成等毒力相关基因，此外，还发现了一些催化酶基因、多种转运蛋白基因和许多能量代谢基因受级联反应控制。我们的数据表明，VirR/VirS-VR-RNA系统是一个全球性的基因调节器，可能控制多种细胞功能，使其在宿主中存活和繁殖，这将成为一种致命的食肉感染。(C)2009爱思唯尔有限公司保留所有权利。
Clostridium perfringens, a Gram-positive anaerobic pathogen, is a causative agent of human gas gangrene that leads to severe rapid tissue destruction and can cause death within hours unless treated immediately. Production of several toxins is known to be controlled by the two-component VirR/VirS system involving a regulatory RNA (VR-RNA) in C. perfringens. To elucidate the precise regulatory network governed by VirR/VirS and VR-RNA, a series of microarray screening using VirR/VirS and VR-RNA-deficient mutants was performed. Finally, by qRT-PCR analysis, 147 genes (30 single genes and 21 putative operons) were confirmed to be under the control of the VirR/VirS-VR-RNA regulatory cascade. Several virulence-related genes for alpha-toxin, kappa-toxin, hyaluronidases, sialidase, and capsular polysaccharide synthesis were found. Furthermore, some genes for catalytic enzymes, various genes for transporters, and many genes for energy metabolism were also found to be controlled by the cascade. Our data indicate that the VirR/VirS-VR-RNA system is a global gene regulator that might control multiple cellular functions to survive and multiply in the host, which would turn out to be a lethal flesh-eating infection. (C) 2009 Elsevier Ltd. All rights reserved.