Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans.
Streptococcus mutans Can Modulate Biofilm Formation and Attenuate the Virulence of Candida albicans.
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DOI：
10.1371/journal.pone.0150457
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Junqueira JC
中科院分区：
文献类型：
--
作者：
Barbosa JO;Rossoni RD;Vilela SF;de Alvarenga JA;Velloso Mdos S;Prata MC;Jorge AO;Junqueira JC
变形链球菌和白色念珠菌同时存在于牙齿表面的口腔生物膜中，但人们对这些物种之间的生态相互作用知之甚少。在这里，我们研究了变形链球菌 UA159 对白色念珠菌生长和致病性的影响。最初，在体外测试了变形链球菌对白色念珠菌生物膜形成和形态发生的影响。接下来，我们使用体内宿主模型研究变形链球菌对白色念珠菌致病性的影响，其中在大蜡蚧幼虫中诱导实验性念珠菌病，并通过存活曲线、血淋巴中白色念珠菌计数和宿主组织中菌丝定量进行分析。在所有测试中，我们评估了变形链球菌细胞的直接影响，以及使用细菌培养滤液由该微生物分泌的副产物的间接影响。体外分析表明，变形链球菌细胞有利于白色念珠菌生物膜的形成。然而，当白色念珠菌与变形链球菌培养滤液接触时，观察到生物膜活细胞减少和菌丝生长抑制。在体内研究中，将变形链球菌细胞或变形链球菌培养物滤液注射到感染了白色念珠菌的大蜡螟幼虫中，增加了这些动物的存活率。此外，当白色念珠菌与变形链球菌培养滤液结合时，在幼虫组织中观察到菌丝形成减少。这些发现表明，变形链球菌可以分泌能够抑制白色念珠菌生物膜形成、形态发生和致病性的子产物，从而减轻大蜡螟模型中的实验性念珠菌病。
Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about the ecological interactions between these species. Here, we studied the effects of S. mutans UA159 on the growth and pathogencity of C. albicans. Initially, the effects of S. mutans on the biofilm formation and morphogenesis of C. albicans were tested in vitro. Next, we investigate the influence of S. mutans on pathogenicity of C. albicans using in vivo host models, in which the experimental candidiasis was induced in G. mellonella larvae and analyzed by survival curves, C. albicans count in hemolymph, and quantification of hyphae in the host tissues. In all the tests, we evaluated the direct effects of S. mutans cells, as well as the indirect effects of the subproducts secreted by this microorganism using a bacterial culture filtrate. The in vitro analysis showed that S. mutans cells favored biofilm formation by C. albicans. However, a reduction in biofilm viable cells and inhibition of hyphal growth was observed when C. albicans was in contact with the S. mutans culture filtrate. In the in vivo study, injection of S. mutans cells or S. mutans culture filtrate into G. mellonella larvae infected with C. albicans increased the survival of these animals. Furthermore, a reduction in hyphal formation was observed in larval tissues when C. albicans was associated with S. mutans culture filtrate. These findings suggest that S. mutans can secrete subproducts capable to inhibit the biofilm formation, morphogenesis and pathogenicity of C. albicans, attenuating the experimental candidiasis in G. mellonella model.