Gastric gland mucin-specific O-glycan expression decreases as tumor cells progress from lobular endocervical gland hyperplasia to cervical mucinous carcinoma, gastric type
Gastric gland mucin-specific O-glycan expression decreases as tumor cells progress from lobular endocervical gland hyperplasia to cervical mucinous carcinoma, gastric type
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DOI：
10.1007/s00428-018-2381-6
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发表时间：
2018-09-01
期刊：
影响因子：
3.5
通讯作者：
Nakayama, Jun
中科院分区：
文献类型：
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作者：
Yamanoi, Kazuhiro;Ishii, Keiko;Nakayama, Jun
胃腺粘蛋白特异性0-聚糖的独特之处在于具有与MUC 6连接的α 1，4-连接的N-乙酰葡糖胺（α GlcNAc）。我们以前报道过在胃和胰腺肿瘤中α GlcNAc相对于MUC 6的表达降低，但其在宫颈腺体病变中的意义尚不清楚。在此，我们分析了9例粘液癌、胃型微小偏离腺癌（GAS-MDA）、5例GAS非MDA（GAS-nonMDA）、14例典型小叶宫颈内腺增生（LEGH）和5例非典型LEGH（共33例病变）中MUC 5AC、MUC 6、α GlcNAc和p16的表达。所有33例均为MUC 5AC阳性。此外，14例典型LEGH、5例非典型LEGH、9例GAS-MDA和5例GAS-non-MDA均为MUC 6阳性。所有14例典型LEGH、2/5例非典型LEGH、3/9例GAS-MDA和1/5例GAS-nonMDA均为α GlcNAc阳性。α-GlcNAc阳性的非典型LEGH或GAS-MDA或GAS-nonMDA病变的比例明显小于典型LEGH病变（分别为P < 0.001和P < 0.01）。在33个病变中，32个为p16阴性。此外，当我们半定量评估所有33个病变中MUC 6和α GlcNAc免疫反应性时，在典型LEGH和GAS-MDA中，α GlcNAc的免疫组织化学评分显著低于MUC 6（P < 0.01）。在典型的LEGH病变中，我们没有观察到α GlcNAc表达相对于MUC 6显著降低。这些研究表明，随着典型LEGH进展为GAS，α GlcNAc表达降低。由于在H.E.中区分MDA和非典型LEGH与典型LEGH是困难的。染色，我们建议α GlcNAc和MUC 6的免疫组织化学分析可能是有用的。
Gastric gland mucin-specific O-glycans are unique in having alpha 1,4-linked N-acetylglucosamine (alpha GlcNAc) attached to MUC6. We previously reported decreased expression of alpha GlcNAc relative to MUC6 in gastric and pancreatic neoplasms, but its significance in cervical glandular lesions remained unclear. Here, we analyzed MUC5AC, MUC6, alpha GlcNAc, and p16 expression in 9 lesions of mucinous carcinoma, gastric type with minimal deviation adenocarcinoma (GAS-MDA), 5 of GAS with nonMDA (GAS-nonMDA), 14 of typical lobular endocervical gland hyperplasia (LEGH), and 5 of atypical LEGH (33 total lesions). All 33 were MUC5AC-positive. Moreover, all 14 typical LEGH, 5 atypical LEGH, 8 of 9 GAS-MDA, and 3 of 5 GAS-nonMDA were MUC6-positive. All 14 typical LEGH, 2 of 5 atypical LEGH, 3 of 9 GAS-MDA, and 1 of 5 GAS-nonMDA were alpha GlcNAc-positive. The proportion of alpha GlcNAc-positive atypical LEGH or GAS-MDA or GAS-nonMDA lesions was significantly smaller than that seen in typical LEGH lesions (P < 0.001 and P < 0.01, respectively). Of 33 lesions, 32 were p16-negative. Furthermore, when we evaluated MUC6 and alpha GlcNAc immunoreactivity semi-quantitatively in all 33 lesions, in typical LEGH and GAS-MDA, the immunohistochemical score for alpha GlcNAc was significantly lower than that for MUC6 (P < 0.01). We did not observe significantly decreased alpha GlcNAc expression relative to MUC6 in typical LEGH lesions. These studies suggest that alpha GlcNAc expression decreases as typical LEGH progresses to GAS. Given the difficulty in distinguishing MDA and atypical LEGH from typical LEGH in H.E. staining, we propose that immunohistochemical analysis of alpha GlcNAc and MUC6 could be useful.