Parathyroid hormone receptors in avian bone cells.
Parathyroid hormone receptors in avian bone cells.
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禽类骨细胞中的甲状旁腺激素受体。
DOI：
10.1073/pnas.79.6.2061
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发表时间：
1982
影响因子：
11.1
通讯作者：
Arnaud,CD
中科院分区：
文献类型：
--
作者：
Pliam,NB;Nyiredy,KO;Arnaud,CD
我们已经证明了在单层培养中合成的牛甲状旁腺激素（1-34）[bPTH-（1-34）]与胚胎禽骨细胞的结合。结合位点具有生理上重要的甲状旁腺激素（PTH）受体的定性和定量特征。在表观稳态（24 ℃下60 min）下，5-10%的电解标记的、受体纯化的125 I标记的bPTH-（1-34）特异性结合细胞，而非特异性结合小于1%的添加的标记激素。Scatchard分析显示单个数量级的PTH结合位点（Kd = 0.6 nM），每个细胞约有10，000个位点。在这种骨细胞系统中，PTH结合到其结合位点，并在相同的浓度范围内刺激cAMP积累。牛PTH-（1-84）以与bPTH-（1-34）相同的表观亲和力与细胞结合。
We have demonstrated binding of synthetic bovine parathyroid hormone (1-34) [bPTH-(1-34)] to embryonic avian bone cells in monolayer culture. The binding sites have qualitative and quantitative characteristics of a physiologically important parathyroid hormone (PTH) receptor. At apparent steady state (60 min at 24 degrees C), 5-10% of electrolytically labeled, receptor-purified 125I-labeled bPTH-(1-34) bound specifically to the cells whereas nonspecific binding was less than 1% of the added labeled hormone. Scatchard analysis showed a single order of PTH binding sites (Kd = 0.6 nM) with approximately 10,000 sites per cell. In this bone cell system, PTH bound to its binding site and stimulated cAMP accumulation over the same concentration range. Bovine PTH-(1-84) bound to the cells with the same apparent affinity as bPTH-(1-34).