LABORATORY INVESTIGATION OF A MULTISTATE FOOD-BORNE OUTBREAK OF ESCHERICHIA-COLI O157-H7 BY USING PULSED-FIELD GEL-ELECTROPHORESIS AND PHAGE TYPING
LABORATORY INVESTIGATION OF A MULTISTATE FOOD-BORNE OUTBREAK OF ESCHERICHIA-COLI O157-H7 BY USING PULSED-FIELD GEL-ELECTROPHORESIS AND PHAGE TYPING
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DOI：
10.1128/jcm.32.12.3013-3017.1994
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发表时间：
1994-12-01
影响因子：
9.4
通讯作者：
GRIFFIN, PM
中科院分区：
文献类型：
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作者：
BARRETT, TJ;LIOR, H;GRIFFIN, PM
采用脉冲凝胶电泳（PFGE）和噬菌体分型对233株大肠杆菌O157:H7进行了分析。所有26株分离株来自与最近多州爆发的O157:H7大肠杆菌感染有关的人，这些人的疾病与食用未煮熟的汉堡有关，所有27株分离株来自大量受污染的汉堡肉，它们具有相同的噬菌体类型和相同的PFGE模式。在疫情爆发前6个月内从华盛顿州获得的74株O157:H7大肠杆菌分离株中有25株和从其他州获得的27株分离株中有10株具有与暴发菌株相同的噬菌体类型，但只有1株分离株具有相同的PFGE模式。因此，PFGE似乎是一种比噬菌体分型更敏感的方法来区分爆发和非爆发相关菌株。7个暴发前散发性分离株和5个暴发期散发性分离株的PFGE模式与暴发菌株的PFGE模式仅相差一个谱带，因此难以确定这些分离株是与暴发有关还是与暴发无关。噬菌体分型和附加酶的PFGE有助于解决这一问题。虽然不像PFGE那样敏感，但噬菌体分型有助于解释PFGE数据，并且可以作为一种简单，快速的筛选方法，以消除对不相关分离株进行PFGE的需要。
Two hundred thirty-three isolates of Escherichia coli O157:H7 were analyzed by both pulsed-held gel electrophoresis (PFGE) and bacteriophage typing. All 26 isolates from persons whose illness was associated with a recent multistate outbreak of E. coli O157:H7 infections linked to the consumption of undercooked hamburgers and all 27 isolates from incriminated lots of hamburger meat had the same phage type and the same PFGE pattern. Twenty-five of 74 E. coli O157:H7 isolates from Washington State and 10 of 27 isolates from other states obtained during the 6 months before the outbreak had the same phage type as the outbreak strain, but only 1 isolate had the same PFGE pattern. PFGE thus appeared to be a more sensitive method than bacteriophage typing for distinguishing outbreak and non-outbreak-related strains. The PFGE patterns of seven preoutbreak sporadic isolates and five sporadic isolates from the outbreak period differed from that of the outbreak strain by a single band, making it difficult to identify these isolates as outbreak or non-outbreak related. Phage typing and PFGE with additional enzymes were helpful in resolving this problem. While not as sensitive as PFGE, phage typing was helpful in interpreting PFGE data and could have been used as a simple, rapid screen to eliminate the need for performing PFGE on unrelated isolates.