A new mouse gene, SRG3, related to the SWI3 of Saccharomyces cerevisiae, is required for apoptosis induced by glucocorticoids in a thymoma cell line
A new mouse gene, SRG3, related to the SWI3 of Saccharomyces cerevisiae, is required for apoptosis induced by glucocorticoids in a thymoma cell line
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DOI：
10.1084/jem.185.10.1827
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发表时间：
1997-05-19
影响因子：
15.3
通讯作者：
Seong, RH
中科院分区：
文献类型：
--
作者：
Jeon, SH;Kang, MG;Seong, RH
我们分离出一个新的小鼠基因，它在胸腺细胞、睾丸和大脑中高度表达。该基因SRG3与酵母转录激活因子SWI3及其人类同源物BAF155具有显著的序列同源性。SRG3编码1100个氨基酸，在三个区域与SWI3蛋白有33-47%的同源性。SRG3蛋白含有一个酸性NH末端，一个myb样DNA结合域，一个亮氨酸拉链基序，以及一个富含脯氨酸和谷氨酰胺的COOH末端区域。兔抗血清作用于SRG3的cooh末端多肽，可识别表观分子质量为155 kD的蛋白。血清还检测到一种170-kD蛋白，似乎是人类BAF170的小鼠同源物。细胞提取物与小鼠SRG3抗血清的免疫沉淀也降低了195-kD蛋白，该蛋白可以被人SWI2蛋白的抗血清识别。结果表明SRG3蛋白与小鼠SWI2相关。SRG3蛋白在胸腺细胞中的表达量约为外周血淋巴细胞的3倍。在胸腺瘤细胞系S49.1中表达SRG3 mRNA的反义RNA，可降低SRG3蛋白的表达水平，减轻糖皮质激素诱导的凋亡细胞死亡。这些结果提示SRG3蛋白参与了糖皮质激素诱导的胸腺瘤细胞系细胞凋亡。这表明SRG3可能在胸腺T细胞发育过程中发挥重要的调节作用。
We isolated a new mouse gene that is highly expressed in thymocytes, testis, and brain. This gene, SRG3, showed a significant sequence homology to SWI3, a yeast transcriptional activator, and its human homolog BAF155. SRG3 encodes 1,100 amino acids and has 33-47% identity with SWI3 protein over three regions. The SRG3 protein contains an acidic NH, terminus, a myb-like DNA binding domain, a leucine-zipper motif, and a proline- and glutamine-rich region at its COOH terminus. Rabbit antiserum raised against a COOH-terminal polypeptide of the SRG3 recognized a protein with an apparent molecular mass of 155 kD. The serum also detected a 170-kD protein that seems to be a mouse homologue of human BAF170. Immuno-precipitation of cell extract with the antiserum against the mouse SRG3 also brought down a 195-kD protein that could be recognized by an antiserum raised against human SWI2 protein. The results suggest that the SRG3 protein associates with a mouse SWI2. The SRG3 protein is expressed about three times higher in thymocytes than in peripheral lymphocytes. The expression of anti-sense RNA to SRG3 mRNA in a thymoma cell line, S49.1, reduced the expression level of the SRG3 protein, and decreased the apoptotic cell death induced by glucocorticoids. These results suggest that the SRG3 protein is involved in the glucocorticoid-induced apoptosis in the thymoma cell line. This implicates that the SRG3 may play an important regulatory role during T cell development in thymus.