Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of Rotaviruses
Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of Rotaviruses
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DOI：
10.1007/s00705-007-0004-7
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发表时间：
2008-03-01
影响因子：
2.7
通讯作者：
Naik, T. N.
中科院分区：
文献类型：
--
作者：
Alam, M. M.;Kobayashi, N.;Naik, T. N.
在轮状病毒SA11株和具有SA11遗传背景的单VP7基因或NSP1基因置换重组体的三个独立感染和多代实验中，分离到3个来自NSP3基因的重排RNA片段的轮状病毒变异株。序列分析表明，这三个重排的NSP3基因具有几乎相同的序列和基因组结构，这些序列和基因组结构是通过在终止密码子之后以头尾方向部分复制开放阅读框来组织的。在三个重排基因中，原始NSP3基因(第一拷贝)和复制部分(第二拷贝)的连接位置是相同的，而没有检测到直接重复，即第一拷贝和第二复制模板之间的同源序列，通常位于连接位置。然而，在第一个拷贝的末尾和第二个拷贝的开始处存在短的相似序列。这些发现提示，NSP3基因的重排可能发生在特定的优先位点，这与3‘-非翻译区和ORF 5’端附近的序列相似性有关。
Three rotavirus variants with a rearranged RNA segment derived from the NSP3 gene were isolated in three independent experiments of coinfection and multiple passages of simian rotavirus strain SA11 and single-VP7-gene- or NSP1-gene-substitution reassortants having genetic background of SA11. Sequence analysis indicated that the three rearranged NSP3 genes had almost identical sequences and genomic structures organized by partial duplication of the open reading frame in a head-to-tail orientation following the termination codon. The junction site of the original NSP3 gene (first copy) and the duplicated portion (second copy) was identical among the three rearranged genes, while a direct repeat, i.e., a homologous sequence between the first copy and second template for duplication, typically located at the junction site, was not detected. However, short similar sequences were present at the end of the first copy and beginning of the second copy. These findings suggest that rearrangement of the NSP3 gene may occur at a certain preferential site which is related to sequence similarity between 3'-untranslated region and a region near the 5'-end of ORF.