Optimization of 3D Alginate Scaffold Properties with Interconnected Porosity Using Freeze-drying Method for Cartilage Tissue Engineering Application
Optimization of 3D Alginate Scaffold Properties with Interconnected Porosity Using Freeze-drying Method for Cartilage Tissue Engineering Application
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DOI：
10.5812/ans.85122
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发表时间：
2019-10-01
影响因子：
0.6
通讯作者：
Ai, Jafar
中科院分区：
文献类型：
--
作者：
Bahrami, Naghmeh;Farzin, Ali;Ai, Jafar
藻酸盐支架因其与细胞外基质的化学相似性而被广泛应用于骨和软骨组织工程中。然而，为了成功地替换硬组织，藻酸盐支架的性能应该量身定做。在本研究中，我们首次通过冷冻干燥的方法优化了藻酸盐支架的孔隙率、降解率、力学性能和成骨性能。采用冷冻干燥法制备了不同浓度(w/v)分别为4、8和16%的藻酸盐支架。对其孔隙率、力学性能、表面润湿行为和成骨性能进行了表征。结果表明，质量分数为8%(w/v)的海藻酸盐支架的孔隙率约为80%，表面亲水，表面与水的接触角为39°+/-0.56，抗压强度为2.7 Mpa。该浓度的藻酸盐在含胎牛血清的Dulbecco‘s改良Eagle’s培养液(DMEM)中的降解率为70%。牙周膜干细胞培养结果证实，8%藻酸盐支架具有良好的生物相容性和细胞分化能力，并能促进细胞向内生长和贴壁。这些结果表明，改性后的8%(w/v)海藻酸盐支架是一种较好的软骨和骨组织替代材料。
Alginate scaffolds are extensively used in bone and cartilage tissue engineering because of their chemical similarity to the extracellular matrix. However, for the successful replacement of hard tissue, the properties of alginate scaffolds should be tailored. In this study, for the first time, we optimized the porosity, degradation rate, mechanical, and osteogenic properties of alginate scaffolds by the freeze-drying method. The freeze-drying method was used to prepare different concentrations of alginate scaffolds (4, 8, and 16% (w/v)). Their porosity, mechanical properties, surface-wetting behavior, and osteogenic properties were characterized. The results showed that the 8% (w/v) alginate scaffold had an interconnected porosity of about 80%, a hydrophilic surface with a contact angle of water on the surface of 39 degrees +/- 0.56, and compressive strength of 2.7 MPa. This concentration of alginate also showed the degradation rate of 70% in Dulbecco's Modified Eagle's Medium (DMEM) with fetal bovine serum. The periodontal ligament stem cells culture results confirmed that the 8% alginate scaffold had good biocompatibility and cell differentiation ability and it could enhance cell ingrowth and attachment. These results showed that the modified 8% (w/v) alginate scaffold is a good candidate in cartilage and bone tissue replacement.