Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature
Cellular thermogenesis compensates environmental temperature fluctuations for maintaining intracellular temperature
复制标题
细胞产热补偿环境温度波动以维持细胞内温度
DOI：
10.1016/j.bbrc.2020.08.110
复制
发表时间：
2020
影响因子：
3.1
通讯作者：
Oka Kotaro
中科院分区：
文献类型：
--
作者：
Yamanaka Ryu;Shindo Yutaka;Hotta Kohji;Hiroi Noriko;Oka Kotaro
温度控制着所有生物分子的状态和动力学，一些细胞过程通常是热源和/或散热器。细胞内温度测量技术的发展使我们能够测量细胞内的温度，为生物学和医学提供了新的视角。然而，很少有人知道，在整个细胞周期的细胞内温度的变化和细胞调节其产热的方式响应于环境温度的波动。在这里，细胞周期依赖的细胞内温度的变化重建从细胞群体的快照在单细胞分辨率使用遍历分析异步培养的HeLa细胞表达的遗传编码的测温。细胞内温度在G1期最高，随着细胞周期的进行沿着逐渐降低，在有丝分裂期突然升高。容易加热的细胞更难冷却，反之亦然，尤其是在G1/S期。总之，细胞内产热取决于细胞周期阶段，并通过补偿环境温度波动来维持细胞内温度。
Temperature governs states and dynamics of all biological molecules, and several cellular processes are often heat sources and/or sinks. Technical achievement of intracellular thermometry enables us to measure intracellular temperature, and it can offer novel perspectives in biology and medicine. However, little is known that changes of intracellular temperature throughout the cell-cycle and the manner of which cells regulates their thermogenesis in response to fluctuation of the environmental temperature. Here, cell-cycle-dependent changes of intracellular temperature were reconstructed from the snapshots of cell population at single-cell resolution using ergodic analysis for asynchronously cultured HeLa cells expressing a genetically encoded thermometry. Intracellular temperature is highest at G1 phase, and it gradually decreases along cell-cycle progression and increases abruptly during mitosis. Cells easily heated up are harder to cool down andvice versa, especially at G1/S phases. Together, intracellular thermogenesis depends on cell-cycle phases and it maintains intracellular temperature through compensating environmental temperature fluctuations.