A stone farm: development of a method for simultaneous production of multiple calcium oxalate stones in vitro
A stone farm: development of a method for simultaneous production of multiple calcium oxalate stones in vitro
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DOI：
10.1007/s00240-003-0379-1
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发表时间：
2004-02-01
影响因子：
--
通讯作者：
Rao, PN
中科院分区：
文献类型：
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作者：
Chow, K;Dixon, J;Rao, PN
我们之前已经展示了如何在体外生长大约1厘米的草酸钙结石。虽然这被证明是一种设计概念，但由于进行比较研究所需的时间，它作为一种实验工具受到了严重限制。在这里，我们描述了这个系统的发展，在这个系统中，六对平行的石材发电机被提供给水，产生一种过饱和的草酸钙介质。在32天的时间里，在无菌准备的人工尿中同时生长了12个结石，从100毫克到大约250毫克。在整个实验过程中，流速、pH和[Ca~(2+)]都是稳定的和可重复性的。叠氮化钠(0.02%)加入到6个结石的生长介质中，使生长速度从5.5 mg/d适度下降到3.4 mg/d。实验设计使得这在视觉上和统计上都很容易检测到(p
We have previously shown how individual calcium oxalate stones of about 1 cm can be grown in vitro. While this proved a design concept, it was severely limited as an experimental tool because of the time required to undertake comparative studies. Here we describe a development of this system in which six parallel pairs of stone generators are supplied with feed solutions generating a medium that is supersaturated with calcium oxalate. Twelve stones were grown simultaneously in aseptically prepared artificial urine over a period of 32 days from 100 mg to about 250 mg. Flow rates, pH and [Ca2+] were stable and reproducible over the course of the experiment. Sodium azide (0.02%) was included in the growth medium of six stones and caused a modest decrease in growth rate from 5.5 to 3.4 mg/day. The experimental design is such that this was readily detectable both visually and statistically (p